Isolation and Molecular Characterization of High Molecular Weight Glutenin Subunit Genes 1Bx13  and 1By16 from Hexaploid Wheat

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	Isolation and Molecular Characterization of High Molecular Weight Glutenin Subunit Genes 1Bx13 and 1By16 from Hexaploid Wheat
	BinShuang Pang; XueYong Zhang
刊名	Journal of Integrative Plant Biology
	2008
卷号	50 期号:3 页码:329-337
关键词	bacterial expression high molecular weight glutenin subunit 1Bx13 1By16 western-blot wheat
ISSN号	1672-9072
英文摘要	The high molecular weight glutenin subunit （HMW-GS） pair 1Bx13＋1By16 are recognized to positively correlate with bread-making quality; however, their molecular data remain unknown. In order to reveal the mechanism by which 1By16 and 1Bx13 creates high quality, their open reading frames （ORFs） were amplified from common wheat Atlas66 and Jimai 20 using primers that were designed based on published sequences of HMW glutenin genes. The ORF of 1By16 was 2220bp, deduced into 738 amino acid residues with seven cysteines including 59 hexapeptides and 22 nanopeptides motifs. The ORF of 1Bx13 was 2385bp, deduced into 795 amino acid residues with four cysteines including 68 hexapeptides, 25 nanopeptides and six tripepUdes motifs. We found that 1By16 was the largest y-type HMW glutenin gene described to date in common wheat. The 1By16 had 36 amino acid residues inserted in the central repetitive domain compared with 1By15. Expression in bacteria and western-blot tests confirmed that the sequence cloned was the ORF of HMW-GS 1By16, and that 1Bx13 was one of the largest 1Bx genes that have been described so far in common wheat, exhibiting a hexapeptide （PGQGQQ） insertion in the end of central repetitive domain compared with 1Bx7. A phylogenetic tree based on the deduced full-length amino acid sequence alignment of the published HMW-GS genes showed that the 1By16 was clustered with Glu-1B-2, and that the 1Bx13 was clustered with Glu-1B-1 alleles.
学科主题	农作物
语种	英语
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/131306]
专题	作物科学研究所
作者单位	Institute of Crop Sciences，Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture, Beijing, 100081
推荐引用方式 GB/T 7714	BinShuang Pang,XueYong Zhang. Isolation and Molecular Characterization of High Molecular Weight Glutenin Subunit Genes 1Bx13 and 1By16 from Hexaploid Wheat[J]. Journal of Integrative Plant Biology,2008,50(3):329-337.
APA	BinShuang Pang,&XueYong Zhang.(2008).Isolation and Molecular Characterization of High Molecular Weight Glutenin Subunit Genes 1Bx13 and 1By16 from Hexaploid Wheat.Journal of Integrative Plant Biology,50(3),329-337.
MLA	BinShuang Pang,et al."Isolation and Molecular Characterization of High Molecular Weight Glutenin Subunit Genes 1Bx13 and 1By16 from Hexaploid Wheat".Journal of Integrative Plant Biology 50.3(2008):329-337.