Identification of Differentially Expressed Genes During Anther Abortion of Taigu Genic Male Sterile Wheat by Combining Suppression Subtractive Hybridization and cDNA Array

CORC > 中国农业科学院 > 作物科学研究所

	Identification of Differentially Expressed Genes During Anther Abortion of Taigu Genic Male Sterile Wheat by Combining Suppression Subtractive Hybridization and cDNA Array
	Qingshan Chang 1; Ronghua Zhou 1; Xiuying Kong 1; Zengliang Yu 2; Jizeng Jia 1
刊名	Journal of Integrative Plant Biology
	2006
卷号	48 期号:11 页码:1348-1354
关键词	anther cDNA array suppression subtractive hybridization Taigu Genic Male Sterile Wheat
ISSN号	1672-9072
英文摘要	Taigu Genic Male Sterile Wheat (TGMSW; Triticum aestivum L.), a dominant genic male sterile germplasm, is of considerable value in the genetic improvement of wheat because of its stable inherence, complete male abortion, and high cross-fertilization rate. To identify specially transcribed genes in sterile anther, a suppression subtractive hybridization (SSH) library was constructed with sterile anther as the tester and fertile anther as the driver. A total of 2 304 SSH inserts amplified by polymerase chain reaction were arrayed using robotic printing. The cDNA arrays were hybridized with 32P-labeled probes prepared from the RNA of forward- and reverse-subtracted anthers. Ninety-six clones were scored as upregulated in sterile anthers compared with the corresponding fertile anthers and some clones were selected for sequencing and analysis in GenBank. Based on their putative functions, 87 non-redundant clones were classified into the following groups: (i) eight genes involved in metabolic processes; (ii) four material transportation genes; (iii) three signal transduction-associated genes; (iv) four stress response and senescence-associated protein genes; (v) seven other functional protein genes; (vi) five genes with no known function; and (vii) another 56 genes with no match to the databases. To test the hybridization efficiency, eight genes were selected and analyzed by Northern blot. The results of the present study provide a comprehensive overview of the genes and gene products involved in anther abortion in TGMSW.
学科主题	植物学
语种	英语
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/131289]
专题	作物科学研究所
作者单位	1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, the National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture, Beijing, 100081; 2.Institute of Plasma Physics, the Chinese Academy of Sciences, Ion Beam Bioengineering Laboratory, CAS, Hefei, 230031
推荐引用方式 GB/T 7714	Qingshan Chang,Ronghua Zhou,Xiuying Kong,et al. Identification of Differentially Expressed Genes During Anther Abortion of Taigu Genic Male Sterile Wheat by Combining Suppression Subtractive Hybridization and cDNA Array[J]. Journal of Integrative Plant Biology,2006,48(11):1348-1354.
APA	Qingshan Chang,Ronghua Zhou,Xiuying Kong,Zengliang Yu,&Jizeng Jia.(2006).Identification of Differentially Expressed Genes During Anther Abortion of Taigu Genic Male Sterile Wheat by Combining Suppression Subtractive Hybridization and cDNA Array.Journal of Integrative Plant Biology,48(11),1348-1354.
MLA	Qingshan Chang,et al."Identification of Differentially Expressed Genes During Anther Abortion of Taigu Genic Male Sterile Wheat by Combining Suppression Subtractive Hybridization and cDNA Array".Journal of Integrative Plant Biology 48.11(2006):1348-1354.