Establishment of a Highly Efficient Regeneration System for the Mature Embryo Culture of Wheat

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	Establishment of a Highly Efficient Regeneration System for the Mature Embryo Culture of Wheat
	Yin Guixiang 1; Wang Yanli 1; She Maoyun 1; Du Lipu 1; Xu Huijun 1; Ma Jingxiu 2; Ye Xingguo 1
刊名	Agricultural Sciences in China
	2011
卷号	10 期号:1 页码:9-17
关键词	wheat mature embryo culture scraping treatment Adi medium highly efficient regeneration
ISSN号	1671-2927
英文摘要	Establishment of a highly efficient regeneration system for the mature embryo of wheat will provide a convenient tool for wheat tissue culture and transformation, thereby facilitating the transformation of foreign genes into wheat. By using the mature embryos derived from 20 different wheat lines including Shi 4185, Yumai 66, Lunxuan 987, CB037, Yangmai 6, Xinchun 9, Bobwhite, Han 6172, Zheng 9023, Jimai 20, Ningchun 4, and Jing 411, the effects of some factors including inoculation methods, initiating culture media, organic additives, antioxidants, and auxins on the regeneration from the explants were evaluated. The results indicated that the scraping embryo culture was better than the whole embryo culture, the Aa medium was better than the SD2 medium and dicamba was better than 2,4-D in increasing the regeneration frequency. An Adi medium was established in this study by adding silver nitrate, cysteine, ascorbic acid, dicamba, glutamine into the Aa medium at the concentration of 4, 40, 100, 2, and 5 mg L-1, respectively. By using the Adi medium and the scraping technique, the regeneration frequencies of the mature embryos of CB037, Lunxuan 987, Han 6172, Yangmai 6, Bobwhite, Zheng 9023, Shi 4185, and Jimai 20 became 85.6, 60.1, 46.0, 42.1, 42.0, 34.0, 33.0, and 32.0%, respectively, which were about 5-8 times higher than that obtained from the conventional culture mediums and techniques. This novel regeneration system could be helpful in wheat transformation.
学科主题	农作物
语种	英语
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/131240]
专题	作物科学研究所
作者单位	1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture;;National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, 100081; 2.Beijing Hybrid Wheat Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100089
推荐引用方式 GB/T 7714	Yin Guixiang,Wang Yanli,She Maoyun,et al. Establishment of a Highly Efficient Regeneration System for the Mature Embryo Culture of Wheat[J]. Agricultural Sciences in China,2011,10(1):9-17.
APA	Yin Guixiang.,Wang Yanli.,She Maoyun.,Du Lipu.,Xu Huijun.,...&Ye Xingguo.(2011).Establishment of a Highly Efficient Regeneration System for the Mature Embryo Culture of Wheat.Agricultural Sciences in China,10(1),9-17.
MLA	Yin Guixiang,et al."Establishment of a Highly Efficient Regeneration System for the Mature Embryo Culture of Wheat".Agricultural Sciences in China 10.1(2011):9-17.