Enthalpy/entropy compensation: Influence of DNA flanking sequence on the binding of 7-amino actinomycin D to its primary binding site in short DNA duplexes

	Enthalpy/entropy compensation: Influence of DNA flanking sequence on the binding of 7-amino actinomycin D to its primary binding site in short DNA duplexes
	Qu XG ; Ren JS ; Riccelli PV ; Benight AS ; Chaires JB
刊名	biochemistry
	2003
卷号	42 期号:41 页码:11960-11967
关键词	TOPOISOMERASE-I INHIBITION CRYSTAL-STRUCTURE PAIR DEOXYOLIGONUCLEOTIDES ENTROPY COMPENSATION MELTING TRANSITIONS OLIGOMER SEQUENCES SMALL MOLECULES HEAT-CAPACITY DRUG-BINDING STRANDED-DNA
ISSN号	0006-2960
通讯作者	qu xg
中文摘要	the effect of the context of the flanking sequence on ligand binding to dna oligonucleotides that contain consensus binding sites was investigated for the binding of the intercalator 7-amino actinomycin d. seven self-complementary dna oligomers each containing a centrally located primary binding site, 5'-a-g-c-t-3', flanked on either side by the sequences (at)(n) or (aa)(n) (with n = 2, 3, 4) and aa(at)(2), were studied. for different flanking sequences, (aa)(n)-series or (at)(n)-series, differential fluorescence enhancements of the ligand due to binding were observed. thermodynamic studies indicated that the flanking sequences not only affected dna stability and secondary structure but also modulated ligand binding to the primary binding site. the magnitude of the ligand binding affinity to the primary site was inversely related to the sequence dependent stability. the enthalpy of ligand binding was directly measured by isothermal titration calorimetry, and this made it possible to parse the binding free energy into its energetic and entropic terms.
收录类别	国际学术会议正式出版物
语种	英语
WOS记录号	WOS:000186007000009
公开日期	2010-08-17
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/15675]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Qu XG,Ren JS,Riccelli PV,et al. Enthalpy/entropy compensation: Influence of DNA flanking sequence on the binding of 7-amino actinomycin D to its primary binding site in short DNA duplexes[J]. biochemistry,2003,42(41):11960-11967.
APA	Qu XG,Ren JS,Riccelli PV,Benight AS,&Chaires JB.(2003).Enthalpy/entropy compensation: Influence of DNA flanking sequence on the binding of 7-amino actinomycin D to its primary binding site in short DNA duplexes.biochemistry,42(41),11960-11967.
MLA	Qu XG,et al."Enthalpy/entropy compensation: Influence of DNA flanking sequence on the binding of 7-amino actinomycin D to its primary binding site in short DNA duplexes".biochemistry 42.41(2003):11960-11967.