Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals

doi:10.1007/s10886-012-0085-y

CORC > 中国农业科学院 > 植物保护研究所

	Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals
	Li, Ke-Ming 1,2; Ren, Li-Yan 1; Zhang, Yong-Jun 1; Wu, Kong-Ming 1; Guo, Yu-Yuan 1
刊名	JOURNAL OF CHEMICAL ECOLOGY
	2012
卷号	38 期号:3 页码:287-294
关键词	Braconidae Electroantennogram Hymenoptera Microplitis mediator Odorant receptor Quantitative real-time polymerase chain reaction (qPCR) Reverse transcription PCR (RT-PCR) analysis RNA interference (RNAi) Walking/flight behavior Y-tube bioassay
ISSN号	0098-0331
DOI	10.1007/s10886-012-0085-y
通讯作者	Li, Ke-Ming
英文摘要	Odorant receptors are thought to play critical roles in the perception of chemosensory stimuli by insects. The primary method to address the functions of odorant receptors in insects is to use in vitro binding assays between the receptors and potential chemical stimuli. We injected MmedOrco dsRNA into the abdominal cavity of a braconid wasp, Microplitis mediator, and assayed for expression of this gene 72 h after treatment (RNAi). Quantitative real-time PCR demonstrated that the level of mRNA expression in MmedOrco dsRNA-treated M. mediator was significantly reduced (> 90%) when compared with water-treated controls. Furthermore, electroantennogram (EAG) responses of M. mediator to two chemical attractants, nonanal and farnesene, were also reduced significantly (similar to 70%) in RNAi-treated M. mediator when compared to controls. RNAi-treated M. mediator also responded by walking/flying at a lower rate to both chemicals when compared with controls in a Y-tube olfactometer bioassay, which provides direct evidence that MmedOrco plays an important role in perception of nonanal and farnesene in M. mediator.
学科主题	Biochemistry & Molecular Biology ; Ecology ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; ECOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000302071700010
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12282]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.NW A&F Univ, Coll Plant Protect, Yangling 712100, Shannxi, Peoples R China
推荐引用方式 GB/T 7714	Li, Ke-Ming,Ren, Li-Yan,Zhang, Yong-Jun,et al. Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals[J]. JOURNAL OF CHEMICAL ECOLOGY,2012,38(3):287-294.
APA	Li, Ke-Ming,Ren, Li-Yan,Zhang, Yong-Jun,Wu, Kong-Ming,&Guo, Yu-Yuan.(2012).Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals.JOURNAL OF CHEMICAL ECOLOGY,38(3),287-294.
MLA	Li, Ke-Ming,et al."Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals".JOURNAL OF CHEMICAL ECOLOGY 38.3(2012):287-294.