Identification of a specific SCAR marker for detection of Tilletia foetida (Wall) Liro pathogen of wheat

doi:10.1134/S1022795412050237

CORC > 中国农业科学院 > 植物保护研究所

	Identification of a specific SCAR marker for detection of Tilletia foetida (Wall) Liro pathogen of wheat
	Zhang, M.1; Chen, W. Q.2; Liu, D.1; Liu, T. G.2; Gao, L.2; Shu, K.1,3
刊名	RUSSIAN JOURNAL OF GENETICS
	2012
卷号	48 期号:6 页码:663-666
ISSN号	1022-7954
DOI	10.1134/S1022795412050237
通讯作者	Zhang, M.
英文摘要	Common bunt is one of the most important destructive diseases of wheat worldwide and is a domestic quarantined disease in China. However, a rapid and efficient method to identify the corresponding pathogens is currently limited. The objective of the present study was to develop a diagnostic molecular marker specific towards Tilletia foetida (Wall) Liro, a causal agent of the bunt disease. One specific DNA fragment for T. foetida (286 bp in length) was amplified using an Amplified Fragment Length Polymorphism (AFLP) assay and, this fragment was cloned and sequenced. One pair of specific primers (SC286-1/SC286-2), which was designed according to the sequence, could specifically amplify the corresponding fragment in all of the T. foetida isolates employed from both the People's Republic of China and United States, whereas this fragment could not be amplified by the other fungal species tested. Therefore, a specific Sequence Characterized Amplified Region (SCAR) marker was developed. This SCAR marker could distinguish T. foetida from related pathogenic fungi efficiently and could be used for the early diagnosis of the common bunt of wheat in the field, and provide an efficient way for disease surveillance and disease forecasting in cereal crop.
学科主题	Genetics & Heredity ; GENETICS & HEREDITY
语种	英语
出版者	MAIK NAUKA/INTERPERIODICA/SPRINGER
WOS记录号	WOS:000305228100013
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12255]
专题	植物保护研究所
作者单位	1.Sichuan Agr Univ, Dept Plant Pathol, Yaan 625014, Sichuan, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhang, M.,Chen, W. Q.,Liu, D.,et al. Identification of a specific SCAR marker for detection of Tilletia foetida (Wall) Liro pathogen of wheat[J]. RUSSIAN JOURNAL OF GENETICS,2012,48(6):663-666.
APA	Zhang, M.,Chen, W. Q.,Liu, D.,Liu, T. G.,Gao, L.,&Shu, K..(2012).Identification of a specific SCAR marker for detection of Tilletia foetida (Wall) Liro pathogen of wheat.RUSSIAN JOURNAL OF GENETICS,48(6),663-666.
MLA	Zhang, M.,et al."Identification of a specific SCAR marker for detection of Tilletia foetida (Wall) Liro pathogen of wheat".RUSSIAN JOURNAL OF GENETICS 48.6(2012):663-666.