Cloning and function analysis of an alfalfa (Medicago sativa L.) zinc finger protein promoter MsZPP

doi:10.1007/s11033-012-1712-y

CORC > 中国农业科学院 > 植物保护研究所

	Cloning and function analysis of an alfalfa (Medicago sativa L.) zinc finger protein promoter MsZPP
	Li, Yan 1; Sun, Yan 2; Yang, Qingchuan 1; Kang, Junmei 1; Zhang, Tiejun 1; Gruber, Margaret Yvonne 3; Fang, Feng 4
刊名	MOLECULAR BIOLOGY REPORTS
	2012
卷号	39 期号:8 页码:8559-8569
关键词	Medicago sativa L. Promoter Deletion analysis Cis-acting elements analysis Transgenic tobacco
ISSN号	0301-4851
DOI	10.1007/s11033-012-1712-y
通讯作者	Li, Yan
英文摘要	A 1272 bp upstream sequence of MsZFN gene was cloned from alfalfa, which was designed as MsZPP (Genbank accession number: FJ 161979.2) using an adaptor-mediated genome walking method. A sole transcription start site was located 69 bp upstream of the translation start site. Its pattern of expression included roots, stem vascular tissues, floral reproductive organs, and leaves, but the promoter did not express in seeds, petals or sepals. Transcription levels can be stimulated by dark, MeJA, and IAA. However, GUS fusion activities had no change by treatments of GA, ABA, drought and high salt for 3 days. Deletion analysis revealed that all sections of the promoter can drive gus gene expression in the root, stem, leaves and floral reproductive organs; however, only fragments longer than the -460 bp promoter can stimulate strong gus gene expression in these organs. In addition, the -460 bp promoter fragment can drive gus expression not only in the vascular tissue, but also in leaf guard cells. The results suggest that the promoter MsZPP plays roles in the regulation of transgene expression, particularly due to its darkness, MeJA, and IAA responsiveness.
学科主题	Biochemistry & Molecular Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000305749200080
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12232]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China 2.China Agr Univ, Inst Grassland Sci, Beijing 100193, Peoples R China 3.Agr & Agri Food Canada, Saskatoon Res Ctr, Saskatoon, SK S7N 0X2, Canada 4.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Li, Yan,Sun, Yan,Yang, Qingchuan,et al. Cloning and function analysis of an alfalfa (Medicago sativa L.) zinc finger protein promoter MsZPP[J]. MOLECULAR BIOLOGY REPORTS,2012,39(8):8559-8569.
APA	Li, Yan.,Sun, Yan.,Yang, Qingchuan.,Kang, Junmei.,Zhang, Tiejun.,...&Fang, Feng.(2012).Cloning and function analysis of an alfalfa (Medicago sativa L.) zinc finger protein promoter MsZPP.MOLECULAR BIOLOGY REPORTS,39(8),8559-8569.
MLA	Li, Yan,et al."Cloning and function analysis of an alfalfa (Medicago sativa L.) zinc finger protein promoter MsZPP".MOLECULAR BIOLOGY REPORTS 39.8(2012):8559-8569.