Cotton gene expression profiles in resistant Gossypium hirsutum cv. Zhongzhimian KV1 responding to Verticillium dahliae strain V991 infection

doi:10.1007/s11033-012-1842-2

CORC > 中国农业科学院 > 植物保护研究所

	Cotton gene expression profiles in resistant Gossypium hirsutum cv. Zhongzhimian KV1 responding to Verticillium dahliae strain V991 infection
	Zhang, Wen-Wei; Jian, Gui-Liang; Jiang, Teng-Fei; Wang, Sheng-Zheng; Qi, Fang-Jun; Xu, Shi-Chang
刊名	MOLECULAR BIOLOGY REPORTS
	2012
卷号	39 期号:10 页码:9765-9774
关键词	Verticillium dahliae Gossypium hirsutum Disease responsive gene SSH libraries Real-time quantitative reverse transcription polymerase chain reaction
ISSN号	0301-4851
DOI	10.1007/s11033-012-1842-2
通讯作者	Zhang, Wen-Wei
英文摘要	Verticillium wilt of cotton (Gossypium hirsutum) is a widespread and destructive disease that is caused by the soil-borne fungus pathogen Verticillium dahliae (V. dahliae). To study the molecular mechanism in wilt tolerance, suppression subtractive hybridization (SSH) and dot blot techniques were used to identify the specifically expressed genes in a superior wilt-resistant cotton cultivar (G. hirsutum cv. Zhongzhimian KV1) after inoculation with pathogen. cDNAs from the root tissues of Zhongzhimian KV1 inoculated with V. dahliae strain V991 or water mock were used to construct the libraries that contain 4800 clones. Based on the results from dot blot analysis, 147 clones were clearly induced by V. dahliae and selected from the SSH libraries for sequencing. A total of 92 up-regulated and 7 down-regulated non-redundant expressed sequences tags (ESTs) were identified as disease responsive genes and classified into 9 functional groups. Two important clues regarding wilt-resistant G. hirsutum were obtained from this study. One was Bet v 1 family; the other was UbI gene family that may play an important role in the defense reaction against Verticillium wilt. The result from real-time quantitative reverse transcription polymerase chain reaction showed that these genes were activated quickly and transiently after inoculation with V. dahliae.
学科主题	Biochemistry & Molecular Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000308251600053
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12219]
专题	植物保护研究所
作者单位	Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wen-Wei,Jian, Gui-Liang,Jiang, Teng-Fei,et al. Cotton gene expression profiles in resistant Gossypium hirsutum cv. Zhongzhimian KV1 responding to Verticillium dahliae strain V991 infection[J]. MOLECULAR BIOLOGY REPORTS,2012,39(10):9765-9774.
APA	Zhang, Wen-Wei,Jian, Gui-Liang,Jiang, Teng-Fei,Wang, Sheng-Zheng,Qi, Fang-Jun,&Xu, Shi-Chang.(2012).Cotton gene expression profiles in resistant Gossypium hirsutum cv. Zhongzhimian KV1 responding to Verticillium dahliae strain V991 infection.MOLECULAR BIOLOGY REPORTS,39(10),9765-9774.
MLA	Zhang, Wen-Wei,et al."Cotton gene expression profiles in resistant Gossypium hirsutum cv. Zhongzhimian KV1 responding to Verticillium dahliae strain V991 infection".MOLECULAR BIOLOGY REPORTS 39.10(2012):9765-9774.