Effects of culture conditions on spore types of Clonostachys rosea 67-1 in submerged fermentation

doi:10.1111/lam.12193

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	Effects of culture conditions on spore types of Clonostachys rosea 67-1 in submerged fermentation
	Sun, M. H.1; Chen, Y. M.1; Liu, J. F.1; Li, S. D.1; Ma, G. Z.2
刊名	LETTERS IN APPLIED MICROBIOLOGY
	2014
卷号	58 期号:4 页码:318-324
关键词	Clonostachys rosea biocontrol agent submerged fermentation conidia chlamydospores
ISSN号	0266-8254
DOI	10.1111/lam.12193
通讯作者	Sun, M. H.
英文摘要	The promising biocontrol isolate Clonostachys rosea 67-1 was investigated to clarify the effects of culture conditions on chlamydospore production in submerged fermentation. Culture conditions significantly affected both performance and types of C.rosea sporulation. C.rosea 67-1 was hard to generate chlamydospores under conventional conditions. However, the proportion of resistant spores increased to 17 center dot 4 and 15 center dot 5% in PD and rice meal media, respectively, in 8days. Chlamydospore productivity was boosted (>threefold) with the addition of 50-200mgl(-1) CuSO4. The pH of the medium played a vital role in 67-1 sporulation. The percentage of chlamydospores decreased rapidly with increased pH (88 center dot 1% at pH 3 center dot 0 to 1 center dot 0% at pH 6 center dot 5). The optimal pH for conidia production was 6 center dot 0-6 center dot 5, at which chlamydospore forming was strongly inhibited. Regulating pH during fermentation contributed to improving output and proportion of resistant spores. When 67-1 was inoculated into broth with an initial pH of 6 center dot 5, followed by adjustment to pH 3 center dot 5 after 48h, the number of chlamydospores reached 1 center dot 1x10(8)ml(-1). The impact of temperature and rotational speed was also analysed; an ultimate capacity of chlamydospores was achieved at 30 degrees C and the speed above 120revmin(-1) (P<0 center dot 05).Significance and Impact of the StudyClonostachys rosea is one of the most promising biocontrol agents in countering many plant fungal diseases. However, large-scale production and commercialization are hampered by the lack of understanding of the impacts of culture conditions on performance and types of C.rosea sporulation and subsequently inadequate research on the techniques for chlamydospore production. In addressing these concerns, this study provides a unique insight into the manipulation of C.rosea sporulation and chlamydospore fermentation of the biocontrol fungus.
学科主题	Biotechnology & Applied Microbiology ; Microbiology ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; MICROBIOLOGY
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000332735500004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11973]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100081, Peoples R China 2.Huaihai Inst Technol & Dev, Lianyungang, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Sun, M. H.,Chen, Y. M.,Liu, J. F.,et al. Effects of culture conditions on spore types of Clonostachys rosea 67-1 in submerged fermentation[J]. LETTERS IN APPLIED MICROBIOLOGY,2014,58(4):318-324.
APA	Sun, M. H.,Chen, Y. M.,Liu, J. F.,Li, S. D.,&Ma, G. Z..(2014).Effects of culture conditions on spore types of Clonostachys rosea 67-1 in submerged fermentation.LETTERS IN APPLIED MICROBIOLOGY,58(4),318-324.
MLA	Sun, M. H.,et al."Effects of culture conditions on spore types of Clonostachys rosea 67-1 in submerged fermentation".LETTERS IN APPLIED MICROBIOLOGY 58.4(2014):318-324.