Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp indica)

doi:10.1371/journal.pone.0099564

CORC > 中国农业科学院 > 植物保护研究所

	Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp indica)
	Deng, Xiao-juan 1,2; Zhang, Hai-qing 1,3; Wang, Yue 1,2; He, Feng 1,4; Liu, Jin-ling 1; Xiao, Xiao 1; Shu, Zhi-feng 1; Li, Wei 5; Wang, Guo-huai 1; Wang, Guo-liang 1,2,4,6
刊名	PLOS ONE
	2014
卷号	9 期号:6 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0099564
通讯作者	Deng, Xiao-juan
英文摘要	Leaf-color is an effective marker to identify the hybridization of rice. Leaf-color related genes function in chloroplast development and the photosynthetic pigment biosynthesis of higher plants. The ygl7 (yellow-green leaf 7) is a mutant with spontaneous yellow-green leaf phenotype across the whole lifespan but with no change to its yield traits. We cloned gene Ygl7 (Os03g59640) which encodes a magnesium-chelatase ChlD protein. Expression of ygl7 turns green-leaves to yellow, whereas RNAi-mediated silence of Ygl7 causes a lethal phenotype of the transgenic plants. This indicates the importance of the gene for rice plant. On the other hand, it corroborates that ygl7 is a non-null mutants. The content of photosynthetic pigment is lower in Ygl7 than the wild type, but its light efficiency was comparatively high. All these results indicated that the mutational YGL7 protein does not cause a complete loss of original function but instead acts as a new protein performing a new function. This new function partially includes its preceding function and possesses an additional feature to promote photosynthesis. Chl1, Ygl98, and Ygl3 are three alleles of the OsChlD gene that have been documented previously. However, mutational sites of OsChlD mutant gene and their encoded protein products were different in the three mutants. The three mutants have suppressed grain output. In our experiment, plant materials of three mutants (ygl7, chl1, and ygl98) all exhibited mutational leaf-color during the whole growth period. This result was somewhat different from previous studies. We used ygl7 as female crossed with chl1 and ygl98, respectively. Both the F-1 and F-2 generation display yellow-green leaf phenotype with their chlorophyll and carotenoid content falling between the values of their parents. Moreover, we noted an important phenomenon: ygl7-NIL's leaf-color is yellow, not yellowy-green, and this is also true of all back-crossed offspring with ygl7.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000337738600042
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11930]
专题	植物保护研究所
作者单位	1.Hunan Agr Univ, Coll Agron, Changsha, Hunan, Peoples R China 2.Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha, Hunan, Peoples R China 3.State Key Lab Hybrid Rice, Wuhan, Hunan, Peoples R China 4.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 5.Hunan Agr Univ, Coll Plant Preservat, Changsha, Hunan, Peoples R China 6.Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA
推荐引用方式 GB/T 7714	Deng, Xiao-juan,Zhang, Hai-qing,Wang, Yue,et al. Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp indica)[J]. PLOS ONE,2014,9(6):-.
APA	Deng, Xiao-juan.,Zhang, Hai-qing.,Wang, Yue.,He, Feng.,Liu, Jin-ling.,...&Wang, Guo-liang.(2014).Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp indica).PLOS ONE,9(6),-.
MLA	Deng, Xiao-juan,et al."Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp indica)".PLOS ONE 9.6(2014):-.