Olfactory co-receptor identification and expression pattern analysis in polyembryonic endoparasitoid Macrocentrus cingulum

doi:10.1016/j.aspen.2015.09.001

CORC > 中国农业科学院 > 植物保护研究所

	Olfactory co-receptor identification and expression pattern analysis in polyembryonic endoparasitoid Macrocentrus cingulum
	Ahmed, Tofael 1,2; Zhang, Tian-tao 1; Wang, Zhen-ying 1; He, Kang-lai 1; Bai, Shu-xiong 1
刊名	JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
	2015
卷号	18 期号:4 页码:719-725
关键词	M. cingulum Orco Olfaction In situ hybridization Gene expression pattern
ISSN号	1226-8615
DOI	10.1016/j.aspen.2015.09.001
通讯作者	Ahmed, Tofael
英文摘要	The wasp, Macrocentrus cingulum, is a polyembryonic endoparasitoid from the insect order Hymenoptera deployed successfully as biological control agent for the control of corn pest insect species from the Lepidopteran genus Ostrinia. As an insect, olfaction plays a crucial role for foraging, host-seeking, mating, ovipositing and avoiding toxic substances. Consequently, the molecular basis of olfaction in this wasp is of particular interest. In an effort to expand our understanding of chemosensory pathways, transcripts encoding the highly conserved insect odorant co-receptor (Orco) were isolated from this wasp. The McinOrco shares high degree of sequence homology with known Orco proteins in other insects. Real-time PCR experiments indicate that the McinOrco is expressed mainly in adult antennae, with expression levels differing by sex. The expressing cells were visualized by adapting a whole mount fluorescence in situ hybridization method. Orco-expressing sensory neurons were shown to be abundant in all 39 segments of male antenna. The highest densities of Orco-expressing cells were identified in segment 20, whereas segment 7 comprised in low level. In case of female antenna, levels of Orco-expressing cells were lower than male antenna. Almost expressed cells were distributed over 40 flagellomeres, with decreasing numbers towards the proximal end. These demonstrate the sexual dimorphism concerning the expression cells of Orco. We are the first to report, Orco in polyembryonic endoparasitoid M. cingulum, which play an important role in detection of olfactory cues in this wasp. (C) 2015 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
学科主题	Entomology
语种	英语
出版者	KOREAN SOC APPLIED ENTOMOLOGY
WOS记录号	WOS:000369349600013
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11601]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China 2.Bangladesh Sugarcane Res Inst, Ishurdi 6620, Pabna, Bangladesh
推荐引用方式 GB/T 7714	Ahmed, Tofael,Zhang, Tian-tao,Wang, Zhen-ying,et al. Olfactory co-receptor identification and expression pattern analysis in polyembryonic endoparasitoid Macrocentrus cingulum[J]. JOURNAL OF ASIA-PACIFIC ENTOMOLOGY,2015,18(4):719-725.
APA	Ahmed, Tofael,Zhang, Tian-tao,Wang, Zhen-ying,He, Kang-lai,&Bai, Shu-xiong.(2015).Olfactory co-receptor identification and expression pattern analysis in polyembryonic endoparasitoid Macrocentrus cingulum.JOURNAL OF ASIA-PACIFIC ENTOMOLOGY,18(4),719-725.
MLA	Ahmed, Tofael,et al."Olfactory co-receptor identification and expression pattern analysis in polyembryonic endoparasitoid Macrocentrus cingulum".JOURNAL OF ASIA-PACIFIC ENTOMOLOGY 18.4(2015):719-725.