Identification of mycoparasitism-related genes in Clonostachys rosea 67-1 active against Sclerotinia sclerotiorum

doi:10.1038/srep18169

CORC > 中国农业科学院 > 植物保护研究所

	Identification of mycoparasitism-related genes in Clonostachys rosea 67-1 active against Sclerotinia sclerotiorum
	Sun, Zhan-Bin; Sun, Man-Hong; Li, Shi-Dong
刊名	SCIENTIFIC REPORTS
	2015
卷号	5 页码:-
ISSN号	2045-2322
DOI	10.1038/srep18169
通讯作者	Sun, Zhan-Bin
英文摘要	Clonostachys rosea is a mycoparasite that has shown great potential in controlling various plant fungal pathogens. In order to find mycoparasitism-related genes in C. rosea, the transcriptome of the efficient isolate 67-1 in association with sclerotia of Sclerotinia sclerotiorum was sequenced and analysed. The results identified 26,351 unigenes with a mean length of 1,102 nucleotides, among which 18,525 were annotated in one or more databases of NR, KEGG, Swiss-Prot, GO and COG. Differentially expressed genes at 8 h, 24 h and 48 h after sclerotial induction were analysed, and 6,890 unigenes were upregulated compared with the control without sclerotia. 713, 1,008 and 1,929 genes were specifically upregulated expressed, while 1,646, 283 and 529 genes were specifically downregulated, respectively. Gene ontology terms analysis indicated that these genes were mainly involved in metabolism of biological process, catalysis of molecular function and cellular component. The expression levels of 12 genes that were upregulated after encountering with S. sclerotiorum were monitored using real-time PCR. The results indicated that the quantitative detection was consistent with the transcriptome analysis. The study provides transcriptional gene expression information on C. rosea parasitizing S. sclerotiorum and forms the basis for further investigation of mycoparasitism-related genes of C. rosea.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000366290300001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11595]
专题	植物保护研究所
作者单位	Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Sun, Zhan-Bin,Sun, Man-Hong,Li, Shi-Dong. Identification of mycoparasitism-related genes in Clonostachys rosea 67-1 active against Sclerotinia sclerotiorum[J]. SCIENTIFIC REPORTS,2015,5:-.
APA	Sun, Zhan-Bin,Sun, Man-Hong,&Li, Shi-Dong.(2015).Identification of mycoparasitism-related genes in Clonostachys rosea 67-1 active against Sclerotinia sclerotiorum.SCIENTIFIC REPORTS,5,-.
MLA	Sun, Zhan-Bin,et al."Identification of mycoparasitism-related genes in Clonostachys rosea 67-1 active against Sclerotinia sclerotiorum".SCIENTIFIC REPORTS 5(2015):-.