Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China | |
Gao Rui; Li Shi-fang; Lu Mei-guang | |
刊名 | JOURNAL OF INTEGRATIVE AGRICULTURE
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2016 | |
卷号 | 15期号:7页码:1667-1671 |
关键词 | Cherry virus A (CVA) genome sequence Capillovirus |
ISSN号 | 2095-3119 |
DOI | 10.1016/S2095-3119(16)61343-6 |
通讯作者 | Gao Rui |
英文摘要 | Cherry virus A (CVA) is a member of the genus Capillovirus, in the family Betaflexiviridae. The infection rate of CVA was high in sweet cherry in China. We determined the complete nucleotide sequences of two isolates of CVA from Tai'an, Shandong Province, China using high fidelity PCR enzymes and specific primer pairs for amplifying long fragments in RT-PCR and RACE. The full-length sequences from isolates ChTA11 and ChTA12 are both 7 382 nucleotide (nt) long, excluding the poly(A) tail, encode two open reading frames (ORFs) and have similar genome organization to the two isolates in Gen-Bank. The complete nucleotide sequence of ChTA11 is 98.2 and 81.2% nt identity to the isolates from Germany and India in GenBank, respectively, and the ChTA12 isolate is 98.2 and 81.0% similar. Analysis of the nucleotide and amino acid sequences showed that the domain of unknown function (DUF1717) is more variable compared with other domains. This is the first report of the complete nucleotide sequences of CVA isolates infecting sweet cherry in China. |
学科主题 | Agriculture, Multidisciplinary |
语种 | 英语 |
出版者 | ELSEVIER SCI LTD |
WOS记录号 | WOS:000380178900025 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/11588] ![]() |
专题 | 植物保护研究所 |
作者单位 | Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China |
推荐引用方式 GB/T 7714 | Gao Rui,Li Shi-fang,Lu Mei-guang. Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2016,15(7):1667-1671. |
APA | Gao Rui,Li Shi-fang,&Lu Mei-guang.(2016).Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China.JOURNAL OF INTEGRATIVE AGRICULTURE,15(7),1667-1671. |
MLA | Gao Rui,et al."Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China".JOURNAL OF INTEGRATIVE AGRICULTURE 15.7(2016):1667-1671. |
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