Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China

doi:10.1016/S2095-3119(16)61343-6

CORC > 中国农业科学院 > 植物保护研究所

	Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China
	Gao Rui; Li Shi-fang; Lu Mei-guang
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2016
卷号	15 期号:7 页码:1667-1671
关键词	Cherry virus A (CVA) genome sequence Capillovirus
ISSN号	2095-3119
DOI	10.1016/S2095-3119(16)61343-6
通讯作者	Gao Rui
英文摘要	Cherry virus A (CVA) is a member of the genus Capillovirus, in the family Betaflexiviridae. The infection rate of CVA was high in sweet cherry in China. We determined the complete nucleotide sequences of two isolates of CVA from Tai'an, Shandong Province, China using high fidelity PCR enzymes and specific primer pairs for amplifying long fragments in RT-PCR and RACE. The full-length sequences from isolates ChTA11 and ChTA12 are both 7 382 nucleotide (nt) long, excluding the poly(A) tail, encode two open reading frames (ORFs) and have similar genome organization to the two isolates in Gen-Bank. The complete nucleotide sequence of ChTA11 is 98.2 and 81.2% nt identity to the isolates from Germany and India in GenBank, respectively, and the ChTA12 isolate is 98.2 and 81.0% similar. Analysis of the nucleotide and amino acid sequences showed that the domain of unknown function (DUF1717) is more variable compared with other domains. This is the first report of the complete nucleotide sequences of CVA isolates infecting sweet cherry in China.
学科主题	Agriculture, Multidisciplinary
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000380178900025
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11588]
专题	植物保护研究所
作者单位	Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Gao Rui,Li Shi-fang,Lu Mei-guang. Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2016,15(7):1667-1671.
APA	Gao Rui,Li Shi-fang,&Lu Mei-guang.(2016).Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China.JOURNAL OF INTEGRATIVE AGRICULTURE,15(7),1667-1671.
MLA	Gao Rui,et al."Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China".JOURNAL OF INTEGRATIVE AGRICULTURE 15.7(2016):1667-1671.