Microbispora camponoti sp nov., a novel actinomycete isolated from the cuticle of Camponotus japonicus Mayr

doi:10.1007/s10482-015-0625-x

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	Microbispora camponoti sp nov., a novel actinomycete isolated from the cuticle of Camponotus japonicus Mayr
	Han, Chuanyu 1; Liu, Chongxi 1; Zhao, Junwei 1,2; Guo, Lifeng 1; Lu, Chang 1; Li, Jiansong 1; Jia, Feiyu 1; Wang, Xiangjing 1; Xiang, Wensheng 1,2
刊名	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
	2016
卷号	109 期号:2 页码:215-223
关键词	Camponotus japonicus Mayr Microbispora camponoti sp nov. Polyphasic taxonomy 16S rRNA gene gyrB gene
ISSN号	0003-6072
DOI	10.1007/s10482-015-0625-x
通讯作者	Han, Chuanyu
英文摘要	A novel actinomycete, designated strain 2C-HV3(T), was isolated from the cuticle of Camponotus japonicus Mayr collected from Harbin, Heilongjiang province, north China and characterised using a polyphasic approach. The 16S rRNA gene sequence of strain 2C-HV3(T) showed that it has high sequence similarities with Microbispora bryophytorum NEAU-TX2-2(T) (99.9 %), Microbispora amethystogenes JCM 3021(T) (98.9 %) and Microbispora rosea subsp. rosea JCM 3006(T) (98.6 %). Phylogenetic analysis based on 16S rRNA and gyrB gene sequences demonstrated that strain 2C-HV3(T) clusters with M. bryophytorum NEAU-TX2-2(T) using two tree-making algorithms. Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain 2C-HV3(T) to the genus Microbispora. Longitudinal paired spores were observed to be born on short sporophores branching from the aerial hyphae. The cell wall was found to contain meso-diaminopimelic acid as the diagnostic diamino acid; madurose was found in the whole cell hydrolysate. The polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside, ninhydrin-positive glycophospholipids, an unidentified phospholipid and an unidentified glycolipid. The predominant menaquinones were identified as MK-9(H-2) and MK-9(H-4). The major fatty acids were identified as 10-methyl C-17:0 and iso-C-16:0. However, a combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain 2C-HV3(T) can be distinguished from its closely related relatives. Consequently, it is proposed that strain 2C-HV3(T) represents a new species of the genus Microbispora, for which the name Microbispora camponoti sp. nov. is proposed. The type strain is 2C-HV3(T) (=CGMCC 4.7281(T) = DSM 100527(T)).
学科主题	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000370135100006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11557]
专题	植物保护研究所
作者单位	1.Northeast Agr Univ, Key Lab Agr Biol Funct Gene Heilongjiang Prov Edu, 59 Mucai St, Harbin 150030, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Han, Chuanyu,Liu, Chongxi,Zhao, Junwei,et al. Microbispora camponoti sp nov., a novel actinomycete isolated from the cuticle of Camponotus japonicus Mayr[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2016,109(2):215-223.
APA	Han, Chuanyu.,Liu, Chongxi.,Zhao, Junwei.,Guo, Lifeng.,Lu, Chang.,...&Xiang, Wensheng.(2016).Microbispora camponoti sp nov., a novel actinomycete isolated from the cuticle of Camponotus japonicus Mayr.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,109(2),215-223.
MLA	Han, Chuanyu,et al."Microbispora camponoti sp nov., a novel actinomycete isolated from the cuticle of Camponotus japonicus Mayr".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 109.2(2016):215-223.