Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.) | |
Huang, Zhaofeng1; Chen, Jinyi1; Zhang, Chaoxian1; Huang, Hongjuan1; Wei, Shouhui1; Zhou, Xinxin2; Chen, Jingchao1; Wang, Xu1 | |
刊名 | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2016 | |
卷号 | 128页码:10-15 |
关键词 | Acetolactate synthase (ALS) Herbicide resistance Imazethapyr Arabidopsis |
ISSN号 | 0048-3575 |
DOI | 10.1016/j.pestbp.2015.10.011 |
通讯作者 | Huang, Zhaofeng |
英文摘要 | Experiments were conducted to confirm imazethapyr resistance in redroot amaranth (Amaranthus retroflexus L.) and study the target-site based mechanism for the resistance. Whole-plant response experiments revealed that the resistant (R) population exhibited 19.16 fold resistance to imazethapyr compared with the susceptible (S) population. In vitro ALS activity assay demonstrated that the imazethapyr I-50 value of the R population was 21.33 times greater than that of the S population. However, qRT-PCR analysis revealed that there is no difference in ALS gene expression between the R and S populations. Sequence analysis revealed an Asp-376-Glu substitution in ALS in the R population. In order to verify that the imazethapyr resistance was conferred by Asp-376-Glu mutation, the ALS-R and ALS-S genes were fused to the CaMV 35S promoter and introduced into Arabidopsis respectively. The expression of ALS-R in transgenic Arabidopsis plants exhibited 13.79 fold resistance to imazethapyr compared to ALS-S transgenic Arabidopsis. (C) 2015 Elsevier Inc. All rights reserved. |
学科主题 | Biochemistry & Molecular Biology ; Entomology ; Physiology |
语种 | 英语 |
出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
WOS记录号 | WOS:000372679700002 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/11539] ![]() |
专题 | 植物保护研究所 |
作者单位 | 1.Chinese Acad Agr Sci, IPP, Key Lab Weed & Rodent Biol & Management, Beijing 100193, Peoples R China 2.Minist Agr, Inst Control Agrochem, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Huang, Zhaofeng,Chen, Jinyi,Zhang, Chaoxian,et al. Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.)[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2016,128:10-15. |
APA | Huang, Zhaofeng.,Chen, Jinyi.,Zhang, Chaoxian.,Huang, Hongjuan.,Wei, Shouhui.,...&Wang, Xu.(2016).Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.).PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,128,10-15. |
MLA | Huang, Zhaofeng,et al."Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.)".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 128(2016):10-15. |
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