Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.)

doi:10.1016/j.pestbp.2015.10.011

CORC > 中国农业科学院 > 植物保护研究所

	Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.)
	Huang, Zhaofeng 1; Chen, Jinyi 1; Zhang, Chaoxian 1; Huang, Hongjuan 1; Wei, Shouhui 1; Zhou, Xinxin 2; Chen, Jingchao 1; Wang, Xu 1
刊名	PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
	2016
卷号	128 页码:10-15
关键词	Acetolactate synthase (ALS) Herbicide resistance Imazethapyr Arabidopsis
ISSN号	0048-3575
DOI	10.1016/j.pestbp.2015.10.011
通讯作者	Huang, Zhaofeng
英文摘要	Experiments were conducted to confirm imazethapyr resistance in redroot amaranth (Amaranthus retroflexus L.) and study the target-site based mechanism for the resistance. Whole-plant response experiments revealed that the resistant (R) population exhibited 19.16 fold resistance to imazethapyr compared with the susceptible (S) population. In vitro ALS activity assay demonstrated that the imazethapyr I-50 value of the R population was 21.33 times greater than that of the S population. However, qRT-PCR analysis revealed that there is no difference in ALS gene expression between the R and S populations. Sequence analysis revealed an Asp-376-Glu substitution in ALS in the R population. In order to verify that the imazethapyr resistance was conferred by Asp-376-Glu mutation, the ALS-R and ALS-S genes were fused to the CaMV 35S promoter and introduced into Arabidopsis respectively. The expression of ALS-R in transgenic Arabidopsis plants exhibited 13.79 fold resistance to imazethapyr compared to ALS-S transgenic Arabidopsis. (C) 2015 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Entomology ; Physiology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000372679700002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11539]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, IPP, Key Lab Weed & Rodent Biol & Management, Beijing 100193, Peoples R China 2.Minist Agr, Inst Control Agrochem, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Huang, Zhaofeng,Chen, Jinyi,Zhang, Chaoxian,et al. Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.)[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2016,128:10-15.
APA	Huang, Zhaofeng.,Chen, Jinyi.,Zhang, Chaoxian.,Huang, Hongjuan.,Wei, Shouhui.,...&Wang, Xu.(2016).Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.).PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,128,10-15.
MLA	Huang, Zhaofeng,et al."Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.)".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 128(2016):10-15.