Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation

doi:10.1111/mpp.12316

CORC > 中国农业科学院 > 植物保护研究所

	Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation
	Huang, Wen-Kun 1,2; Ji, Hong-Li 1,3; Gheysen, Godelieve 1; Kyndt, Tina 1
刊名	MOLECULAR PLANT PATHOLOGY
	2016
卷号	17 期号:4 页码:614-624
关键词	callose hydrogen peroxide induced defence lignin Meloidogyne graminicola priming thiamine
ISSN号	1464-6722
DOI	10.1111/mpp.12316
通讯作者	Huang, Wen-Kun
英文摘要	Thiamine (vitamin B1, VB1) can act as a plant defence trigger, or priming agent, leading to a rapid counterattack on pathogen invasion. In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated. Thiamine treatment and subsequent nematode inoculation activated hydrogen peroxide (H2O2) accumulation and lignin deposition in plant roots, and this correlated with enhanced transcription of OsPAL1 and OsC4H, two genes involved in the phenylpropanoid pathway. The number of nematodes in rice roots was slightly but significantly reduced, and the development of the nematodes was delayed, whereas no direct toxic effects of VB1 on nematode viability and infectivity were observed. The combined application of thiamine with l-2-aminooxy-3-phenylpropionic acid (AOPP), an inhibitor of phenylalanine ammonia-lyase (PAL), significantly hampered the VB1-priming capacity. These findings indicate that thiamine-induced priming in rice involves H2O2 and phenylpropanoid-mediated lignin production, which hampers nematode infection. Further cellular and molecular studies on the mechanism of thiamine-induced defence will be useful for the development of novel nematode control strategies.
学科主题	Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000374693400013
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11494]
专题	植物保护研究所
作者单位	1.Univ Ghent, Dept Mol Biotechnol, Coupure Links 653, B-9000 Ghent, Belgium 2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 3.Sichuan Acad Agr Sci, Inst Plant Protect, Jingjusi Rd 20, Chengdu 610066, Peoples R China
推荐引用方式 GB/T 7714	Huang, Wen-Kun,Ji, Hong-Li,Gheysen, Godelieve,et al. Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation[J]. MOLECULAR PLANT PATHOLOGY,2016,17(4):614-624.
APA	Huang, Wen-Kun,Ji, Hong-Li,Gheysen, Godelieve,&Kyndt, Tina.(2016).Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation.MOLECULAR PLANT PATHOLOGY,17(4),614-624.
MLA	Huang, Wen-Kun,et al."Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation".MOLECULAR PLANT PATHOLOGY 17.4(2016):614-624.