Tier-1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non-target arthropods

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	Tier-1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non-target arthropods
	Li, Yun-He 1; Romeis, Joerg 1,2; Wu, Kong-Ming 1; Peng, Yu-Fa 1
刊名	Insect Science
	2014
卷号	21 期号:2 页码:125-134
关键词	artificial diet Cry protein environmental risk assessment ELISA positive control sensitive insect bioassay
ISSN号	1672-9609
英文摘要	In assessing an insect-resistant genetically engineered (IRGE) crop before its commercialization, researchers normally use so-called “Tier-1 assays” as the initial step to determine the effects of the crop on non-target organisms. In these tests, the insecticidal proteins (IPs) produced by the IRGEs are added to the diets of test organisms in the laboratory. Test organisms in such assays can be directly exposed to much higher concentrations of the test IPs than they would encounter in the field. The results of Tier-1 assays are thus more conservative than those generated in studies in which the organisms are exposed to the IPs by feeding on IRGE plant tissue or in the case of predators or parasites, by feeding on invertebrate prey or hosts that have fed on IRGE plant tissue. In this report, we consider three important factors that must be considered in Tier-1 assays: (i) methods for delivery of the IP to the test organisms; (ii) the need for and selection of compounds used as positive controls; and (iii) methods for monitoring the concentration, stability and bioactivity of the IP during the assay. We also analyze the existing data from Tier-1 assays regarding the toxicity of Bt Cry proteins to non-target arthropod species. The data indicate that the widely used Bt proteins have no direct toxicity to non-target organisms.
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11121]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.Agroscope Reckenholz Tanikon Res Stn ART, Zurich, Switzerland
推荐引用方式 GB/T 7714	Li, Yun-He,Romeis, Joerg,Wu, Kong-Ming,et al. Tier-1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non-target arthropods[J]. Insect Science,2014,21(2):125-134.
APA	Li, Yun-He,Romeis, Joerg,Wu, Kong-Ming,&Peng, Yu-Fa.(2014).Tier-1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non-target arthropods.Insect Science,21(2),125-134.
MLA	Li, Yun-He,et al."Tier-1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non-target arthropods".Insect Science 21.2(2014):125-134.