Use of a dietary exposure system for screening of insecticidal compounds for their toxicity to the planthopper Laodelphax striatellus

CORC > 中国农业科学院 > 植物保护研究所

	Use of a dietary exposure system for screening of insecticidal compounds for their toxicity to the planthopper Laodelphax striatellus
	Wang Zengxia 1; Lin Kejian 1; Romeis Jorg 1; Liu Yonglei 1; Liu Zewen 2; Li Yunhe 1; Peng Yufa 1
刊名	Insect Science
	2014
卷号	21 期号:5 页码:667-675
关键词	Cry2Aa E-64 genetically engineered rice potassium arsenate risk assessment
ISSN号	1672-9609
英文摘要	We developed a dietary exposure assay for screening insecticidal compounds for their toxicity and for assessing the side effects of insecticidal proteins produced by genetically engineered (GE) plants on the planthopper Laodelphax striatellus Fallen. The fitness bioassay confirmed that the diet fulfills the requirements to be used in the dietary exposure system. To validate the efficacy of the dietary exposure system, nymphs of L.striatellus were fed diets treated with different concentrations of an inorganic stomach poison, potassium arsenate (PA), or a cysteine protease inhibitor, E-64. The results showed that with increasing concentrations of E-64, the larval development time was prolonged, the adult weight was reduced and the survival rate of L. striatellus was decreased. Similarly the survival rates of L. striatellus consistently decreased with increasing PA content in the diet. The data indicate that the dietary exposure assay is able to detect the effects of insecticidal compounds on L. striatellus. Subsequently, this assay was successfully used for assessing the potential toxicity of Cry2Aa. The results showed that L. striatellus larvae were not negatively affected when fed the artificial diet containing purified Cry2Aa at 300 μg/g diet. In the assay, the stability and bioactivity of crystal (Cry) proteins in the food sources were confirmed by enzyme-linked immunosorbent assay and sensitive-insect bioassays. These results show that L. striatellus is not sensitive to Cry2Aa. We conclude that the dietary exposure system is valid and useful for assessing the toxicity of insecticidal compounds produced by GE plants on planthoppers.
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11102]
专题	植物保护研究所
作者单位	1.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 2.Nanjing Agricultural University, Nanjing
推荐引用方式 GB/T 7714	Wang Zengxia,Lin Kejian,Romeis Jorg,et al. Use of a dietary exposure system for screening of insecticidal compounds for their toxicity to the planthopper Laodelphax striatellus[J]. Insect Science,2014,21(5):667-675.
APA	Wang Zengxia.,Lin Kejian.,Romeis Jorg.,Liu Yonglei.,Liu Zewen.,...&Peng Yufa.(2014).Use of a dietary exposure system for screening of insecticidal compounds for their toxicity to the planthopper Laodelphax striatellus.Insect Science,21(5),667-675.
MLA	Wang Zengxia,et al."Use of a dietary exposure system for screening of insecticidal compounds for their toxicity to the planthopper Laodelphax striatellus".Insect Science 21.5(2014):667-675.