A de novo transcriptomic analysis to reveal functional genes in Apolygus lucorum

doi:10.1111/1744-7917.12188

CORC > 中国农业科学院 > 植物保护研究所

	A de novo transcriptomic analysis to reveal functional genes in Apolygus lucorum
	Cao, De-Pan 1,2; Liu, Yang 1; Wei, Jin-Jin 1; Li, Jian-Hong 1,2; Wang, Gui-Rong 1
刊名	Insect Science
	2016
卷号	23 期号:1 页码:2-14
关键词	Apolygus lucorum digestion immunity metamorphosis transcriptome
ISSN号	1672-9609
DOI	10.1111/1744-7917.12188
英文摘要	The widespread planting of genetically engineered cotton producing the Cry1Ac toxin has led to significantly reduced pesticide applications since 1997. However, consequently, the number of green mirid bugs (GMB), Apolygus lucorum (Meyer-Dur) has increased. So far the GMB, instead of the cotton bollworm Helicoverpa armigera (Hubner), has become the major pest in the transgenic Bt cotton field and has influenced cotton yield. Disproportionately, only a few studies on GMB at molecular level have been reported. Libraries from both third instar nymphs and adults were sequenced using Illumina technology, producing more than 106 million short reads and assembled into 63 029 unigenes of mean length 597 nt and N50 813 nt, ranging from 300 nt to 9771 nt. BLASTx analysis against Nr, Swissprot, GO and COG was performed to annotate these unigenes. As a result, 26 478 unigenes (42.01%) matched to known proteins and 107 immune-related, 320 digestive-related and 53 metamorphosis-related geneswere detected in these annotated unigenes. Additionally, we profiled gene expression using mapping based differentially expressed genes (DEGs) strategy between the two developmental stages: nymph and adult. The results demonstrated that thousands of genes were significantly differentially expressed at different developmental stages. The transcriptome and gene expression data provided comprehensive and global gene resources of GMB. This transcriptome would improve our understanding of the molecular mechanisms of various underlying biological characteristics, including development, digestion and immunity in GMB. Therefore, these findings could help elucidate the intrinsic factors of the GMB resurgence, offering novel pest management targets for future transgenic cotton breeding.
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11062]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.Huazhong Agr Univ, Coll Plant Sci & Technol, Lab Pesticide, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Cao, De-Pan,Liu, Yang,Wei, Jin-Jin,et al. A de novo transcriptomic analysis to reveal functional genes in Apolygus lucorum[J]. Insect Science,2016,23(1):2-14.
APA	Cao, De-Pan,Liu, Yang,Wei, Jin-Jin,Li, Jian-Hong,&Wang, Gui-Rong.(2016).A de novo transcriptomic analysis to reveal functional genes in Apolygus lucorum.Insect Science,23(1),2-14.
MLA	Cao, De-Pan,et al."A de novo transcriptomic analysis to reveal functional genes in Apolygus lucorum".Insect Science 23.1(2016):2-14.