The characterization of GmTIP, a root-specific gene from soybean, and the expression analysis of its promoter

doi:10.1007/s11240-014-0682-2

	The characterization of GmTIP, a root-specific gene from soybean, and the expression analysis of its promoter
	Chen, Li; Jiang, Bingjun; Wu, Cunxiang; Sun, Shi; Hou, Wensheng; Han, Tianfu
刊名	PLANT CELL TISSUE AND ORGAN CULTURE
	2015
卷号	121 期号:2 页码:259-274
关键词	Soybean GmTIP Promoter GUS Root-specific Deletion
ISSN号	0167-6857
DOI	10.1007/s11240-014-0682-2
通讯作者	Chen, Li
英文摘要	The soybean encounters severe root-related biotic and abiotic stresses causing extensive yield losses annually. The use of a root-specific promoter to confer expression of genes in a targeted manner has the potential to benefit the genetic improvement of the soybean. Several tonoplast intrinsic protein genes (TIPs) in plants like tobacco, strawberry, tomato, maize, pine, have been identified exhibiting root-specific expression. To explore the possibility of root-specific gene and promoter utilizations in soybean, we cloned soybean GmTIP and the upstream promoter sequence from soybean, and examined the expression patterns and promoter activity. Fluorometric GUS assays revealed that the GUS activity was obviously higher in roots than in leaves in transgenic Arabidopsis, regardless of the length of the promoter fragment. Weak GUS expression was found in other organs except root in transgenic Arabidopsis with the GmTIPp-2054 construct. Deletion analysis of the GmTIP promoter displayed that it still keep root-specific expression and can also be induced by various factors, such as methyl jasmonate, salicylic acid, abscisic acid, gibberellins acid, indole-3-acetic acid, NaCl, and PEG6000. This study provides a useful tool for soybean root improvement by genetic engineering.
学科主题	Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000352652800001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4722]
专题	作物科学研究所_转基因中心
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, MOA Key Lab Soybean Biol Beijing, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Chen, Li,Jiang, Bingjun,Wu, Cunxiang,et al. The characterization of GmTIP, a root-specific gene from soybean, and the expression analysis of its promoter[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2015,121(2):259-274.
APA	Chen, Li,Jiang, Bingjun,Wu, Cunxiang,Sun, Shi,Hou, Wensheng,&Han, Tianfu.(2015).The characterization of GmTIP, a root-specific gene from soybean, and the expression analysis of its promoter.PLANT CELL TISSUE AND ORGAN CULTURE,121(2),259-274.
MLA	Chen, Li,et al."The characterization of GmTIP, a root-specific gene from soybean, and the expression analysis of its promoter".PLANT CELL TISSUE AND ORGAN CULTURE 121.2(2015):259-274.