A Wheat Cinnamyl Alcohol Dehydrogenase TaCAD12 Contributes to Host Resistance to the Sharp Eyespot Disease

doi:10.3389/fpls.2016.01723

	A Wheat Cinnamyl Alcohol Dehydrogenase TaCAD12 Contributes to Host Resistance to the Sharp Eyespot Disease
	Rong, Wei; Luo, Meiying; Shan, Tianlei; Wei, Xuening; Du, Lipu; Xu, Huijun; Zhang, Zengyan
刊名	FRONTIERS IN PLANT SCIENCE
	2016
卷号	7 页码:-
关键词	hexaploid wheat cinnamyl alcohol dehydrogenase (CAD) Rhizoctonia cerealis sharp eyespot resistance defense genes monolignol biosynthesis-related genes
ISSN号	1664-462X
DOI	10.3389/fpls.2016.01723
通讯作者	Rong, Wei
英文摘要	Sharp eyespot, caused mainly by the necrotrophic fungus Rhizoctonia cerealis, is a destructive disease in hexaploid wheat (Triticum aestivum L.). In Arabidopsis, certain cinnamyl alcohol dehydrogenases (CADs) have been implicated in monolignol biosynthesis and in defense response to bacterial pathogen infection. However, little is known about CADs in wheat defense responses to necrotrophic or soil-borne pathogens. In this study, we isolate a wheat CAD gene TaCAD12 in response to R. cerealis infection through microarray-based comparative transcriptomics, and study the enzyme activity and defense role of TaCAD12 in wheat. The transcriptional levels of TaCAD12 in sharp eyespot-resistant wheat lines were significantly higher compared with those in susceptible wheat lines. The sequence and phylogenetic analyses revealed that TaCAD12 belongs to IV group in CAD family. The biochemical assay proved that TaCAD12 protein is an authentic CAD enzyme and possesses catalytic efficiencies toward both coniferyl aldehyde and sinapyl aldehyde. Knock-down of TaCAD12 transcript significantly repressed resistance of the gene-silenced wheat plants to sharp eyespot caused by R. cerealis, whereas TaCAD12 overexpression markedly enhanced resistance of the transgenic wheat lines to sharp eyespot. Furthermore, certain defense genes (Defensin, PR10, PR17c, and Chitinase1) and monolignol biosynthesis-related genes (TaCAD1, TaCCR, and TaCOMT1) were up-regulated in the TaCAD/2-overexpressing wheat plants but down-regulated in TaCAD12-silencing plants. These results suggest that TaCAD12 positively contributes to resistance against sharp eyespot through regulation of the expression of certain defense genes and monolignol biosynthesis related genes in wheat.
学科主题	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000387819000001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105289]
专题	作物科学研究所_分子生物学系
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facility Crop Gene Resources & Genet Imp, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Rong, Wei,Luo, Meiying,Shan, Tianlei,et al. A Wheat Cinnamyl Alcohol Dehydrogenase TaCAD12 Contributes to Host Resistance to the Sharp Eyespot Disease[J]. FRONTIERS IN PLANT SCIENCE,2016,7:-.
APA	Rong, Wei.,Luo, Meiying.,Shan, Tianlei.,Wei, Xuening.,Du, Lipu.,...&Zhang, Zengyan.(2016).A Wheat Cinnamyl Alcohol Dehydrogenase TaCAD12 Contributes to Host Resistance to the Sharp Eyespot Disease.FRONTIERS IN PLANT SCIENCE,7,-.
MLA	Rong, Wei,et al."A Wheat Cinnamyl Alcohol Dehydrogenase TaCAD12 Contributes to Host Resistance to the Sharp Eyespot Disease".FRONTIERS IN PLANT SCIENCE 7(2016):-.