W55a Encodes a Novel Protein Kinase That Is Involved in Multiple Stress Responses

doi:10.1111/j.1744-7909.2008.00776.x

	W55a Encodes a Novel Protein Kinase That Is Involved in Multiple Stress Responses
	Xu, Zhao-Shi 1; Liu, Li 1,2; Ni, Zhi-Yong 1; Liu, Pei 1; Chen, Ming 1; Li, Lian-Cheng 1; Chen, Yao-Feng 2; Ma, You-Zhi 1
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2009
卷号	51 期号:1 页码:58-66
关键词	chromosomal location induction kinetics SnRK2 stress response transgenic Arabidopsis wheat
ISSN号	1672-9072
DOI	10.1111/j.1744-7909.2008.00776.x
通讯作者	Xu, Zhao-Shi
英文摘要	Protein kinases play crucial roles in response to external environment stress signals. A putative protein kinase, W55a, belonging to SNF1-related protein kinase 2 (SnRK2) subfamily, was isolated from a cDNA library of drought-treated wheat seedlings. The entire length of W55a was obtained using rapid amplification of 5' cDNA ends (5'-RACE) and reverse transcription-polymerase chain reaction(RT-PCR). It contains a 1 029 -bp open reading frame (ORF) encoding 342 amino acids. The deduced amino acid sequence of W55a had eleven conserved catalytic subdomains and one Ser/Thr protein kinase active-site that characterize Ser/Thr protein kinases. Phylogenetic analysis showed that W55a was 90.38% homologous with rice SAPK1, a member of the SnRK2 family. Using nullisomic-tetrasomic and ditelocentric lines of Chinese Spring, W55a was located on chromosome 2BS. Expression pattern analysis revealed that W55a was upregulated by drought and salt, exogenous abscisic acid, salicylic acid, ethylene and methyl jasmonate, but was not responsive to cold stress. In addition, W55a transcripts were abundant in leaves, but not in roots or stems, under environmental stresses. Transgenic Arabidopsis plants overexpressing W55a exhibited higher tolerance to drought. Based on these findings, W55a encodes a novel dehydration-responsive protein kinase that is involved in multiple stress signal transductions.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; PLANT SCIENCES
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000262106300009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5548]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Genet & Breeding, Minist Agr,Natl Key Facil Crop Gene Resources & G, Beijing 100081, Peoples R China 2.NW A&F Univ, Agron Coll, Yangling 712100, Peoples R China
推荐引用方式 GB/T 7714	Xu, Zhao-Shi,Liu, Li,Ni, Zhi-Yong,et al. W55a Encodes a Novel Protein Kinase That Is Involved in Multiple Stress Responses[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2009,51(1):58-66.
APA	Xu, Zhao-Shi.,Liu, Li.,Ni, Zhi-Yong.,Liu, Pei.,Chen, Ming.,...&Ma, You-Zhi.(2009).W55a Encodes a Novel Protein Kinase That Is Involved in Multiple Stress Responses.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,51(1),58-66.
MLA	Xu, Zhao-Shi,et al."W55a Encodes a Novel Protein Kinase That Is Involved in Multiple Stress Responses".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 51.1(2009):58-66.