Establishment of a Gene Expression System in Rice Chloroplast and Obtainment of PPT-Resistant Rice Plants

doi:10.1016/S1671-2927(08)60259-X

	Establishment of a Gene Expression System in Rice Chloroplast and Obtainment of PPT-Resistant Rice Plants
	Li Yi-nue 1; Sun Bing-yao 2; Su Ning 3; Meng Xiang-xun 2; Zhang Zhi-fang 1; Shen Gui-fang 1
刊名	AGRICULTURAL SCIENCES IN CHINA
	2009
卷号	8 期号:6 页码:643-651
关键词	Oryza saliva L. chloroplast transformation bar gene homologous fragments
ISSN号	1671-2927
DOI	10.1016/S1671-2927(08)60259-X
通讯作者	Li Yi-nue
英文摘要	In contrast to the situation of random integration of foreign genes in nuclear transformation, the introduction of genes via chloroplast genetic engineering is characterized by site-specific pattern via homologous recombination. To establish an expression system for alien genes in rice chloroplast, the intergenic region of ndhF and trnL was selected as target for sitespecific integration of PPT-resistant bar gene in this study. Two DNA fragments suitable for homologous recombination were cloned from rice chloroplast genome DNA using PCR technique, and the chloroplast-specific expression vector pRB was constructed by fusing a modified 16S rRNA gene promoter to bar gene together with terminator of psbA gene 3' sequence. Chloroplast transformation was carried out by biolistic bombardment of sterile rice calli with the pRB construct. Subsequently, the regenerated plantlets and seeds of progeny arising from reciprocal cross to the wild-type lines were obtained. Molecular analysis suggested that the bar gene has been integrated into rice chloroplast genome. Genetic analysis revealed that bar gene could be transmitted and expressed normally in chloroplast genome. Thus, the bar gene conferred not only selection pressure for the transformation of rice chloroplast genome, but PPT-resistant trait for rice plants as well. It is suggested that an efficient gene expression system in the rice chloroplast has been established by chloroplast transformation technique.
学科主题	Agriculture, Multidisciplinary ; AGRICULTURE, MULTIDISCIPLINARY
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000278076000001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5513]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China 2.Suzhou Univ, Coll Life Sci, Suzhou 215123, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Li Yi-nue,Sun Bing-yao,Su Ning,et al. Establishment of a Gene Expression System in Rice Chloroplast and Obtainment of PPT-Resistant Rice Plants[J]. AGRICULTURAL SCIENCES IN CHINA,2009,8(6):643-651.
APA	Li Yi-nue,Sun Bing-yao,Su Ning,Meng Xiang-xun,Zhang Zhi-fang,&Shen Gui-fang.(2009).Establishment of a Gene Expression System in Rice Chloroplast and Obtainment of PPT-Resistant Rice Plants.AGRICULTURAL SCIENCES IN CHINA,8(6),643-651.
MLA	Li Yi-nue,et al."Establishment of a Gene Expression System in Rice Chloroplast and Obtainment of PPT-Resistant Rice Plants".AGRICULTURAL SCIENCES IN CHINA 8.6(2009):643-651.