Molecular characterization of GmNHX2, a Na+/H+ antiporter gene homolog from soybean, and its heterologous expression to improve salt tolerance in Arabidopsis

doi:10.1007/s11434-009-0564-x

	Molecular characterization of GmNHX2, a Na+/H+ antiporter gene homolog from soybean, and its heterologous expression to improve salt tolerance in Arabidopsis
	Zhou GuoAn; Guan RongXia; Li YingHui; Chang RuZhen; Qiu LiJuan
刊名	CHINESE SCIENCE BULLETIN
	2009
卷号	54 期号:19 页码:3536-3545
关键词	GmNHX2 Na+/H+ antiporter salt tolerance soybean ion homeostasis
ISSN号	1001-6538
DOI	10.1007/s11434-009-0564-x
通讯作者	Zhou GuoAn
英文摘要	Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. Here, a putative Na+/H+ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCl and polyethylene glycol (PEG) treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells. Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na+/H+ antiporter and may function to regulate ion homeostasis under salt stress.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000271025500021
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5488]
专题	作物科学研究所_分子生物学系
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Germplasm & Biotechnol MOA, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhou GuoAn,Guan RongXia,Li YingHui,et al. Molecular characterization of GmNHX2, a Na+/H+ antiporter gene homolog from soybean, and its heterologous expression to improve salt tolerance in Arabidopsis[J]. CHINESE SCIENCE BULLETIN,2009,54(19):3536-3545.
APA	Zhou GuoAn,Guan RongXia,Li YingHui,Chang RuZhen,&Qiu LiJuan.(2009).Molecular characterization of GmNHX2, a Na+/H+ antiporter gene homolog from soybean, and its heterologous expression to improve salt tolerance in Arabidopsis.CHINESE SCIENCE BULLETIN,54(19),3536-3545.
MLA	Zhou GuoAn,et al."Molecular characterization of GmNHX2, a Na+/H+ antiporter gene homolog from soybean, and its heterologous expression to improve salt tolerance in Arabidopsis".CHINESE SCIENCE BULLETIN 54.19(2009):3536-3545.