Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.) | |
He, Junguang2; Dong, Zhigang2; Jia, Zhiwei2; Wang, Jianhua3; Wang, Guoying1 | |
刊名 | MOLECULAR BIOLOGY REPORTS |
2010 | |
卷号 | 37期号:2页码:865-874 |
关键词 | RdRP SCMV SA Maize |
ISSN号 | 0301-4851 |
DOI | 10.1007/s11033-009-9692-2 |
通讯作者 | He, Junguang |
英文摘要 | RNA-dependent RNA polymerases (RdRPs) in plants have been reported to be involved in post-transcriptional gene silencing (PTGS) and antiviral defense. In this report, an RdRP gene from maize (ZmRdRP1) was obtained by rapid amplification of cDNA ends (RACE) and RT-PCR. The mRNA of ZmRdRP1 was composed of 3785 nucleotides, including a 167 nt 5' untranslated region (UTR), a 291 nt 3'UTR and a 3327 nt open reading frame (ORF), which encodes a putative protein of 1108 amino acids with an estimated molecular mass of 126.9 kDa and a predicated isoelectric point (pI) of 8.37. Real-time quantitative RT-PCR analysis showed that ZmRdRP1 was elicited by salicylic acid (SA) treatment, methyl jasmonate (MeJA) treatment and sugarcane mosaic virus (SCMV) infection. We silenced ZmRdRP1 by constitutively expressing an inverted-repeat fragment of ZmRdRP1 (ir-RdRP1) in transgenic maize plants. Further studies revealed that the ir-RdRP1 transgenic plants were more susceptible to SCMV infection than wild type plants. Virus-infected transgenic maize plants developed more serious disease symptoms and accumulated more virus than wild type plants. These findings suggested that ZmRdRP1 was involved in antiviral defense in maize. |
学科主题 | Biochemistry & Molecular Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000273085800029 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/5453] |
专题 | 作物科学研究所_分子生物学系 |
作者单位 | 1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.China Agr Univ, State Key Lab Agrobiotecnol, Coll Biol Sci, Beijing 100193, Peoples R China 3.China Agr Univ, Coll Agr & Biotechnol, Beijing 100193, Peoples R China |
推荐引用方式 GB/T 7714 | He, Junguang,Dong, Zhigang,Jia, Zhiwei,et al. Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.)[J]. MOLECULAR BIOLOGY REPORTS,2010,37(2):865-874. |
APA | He, Junguang,Dong, Zhigang,Jia, Zhiwei,Wang, Jianhua,&Wang, Guoying.(2010).Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.).MOLECULAR BIOLOGY REPORTS,37(2),865-874. |
MLA | He, Junguang,et al."Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.)".MOLECULAR BIOLOGY REPORTS 37.2(2010):865-874. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论