Isolation and characterization of an AGAMOUS-like gene from Hosta plantaginea

doi:10.1007/s11033-011-1047-0

	Isolation and characterization of an AGAMOUS-like gene from Hosta plantaginea
	Wang, Ying 1,2; Zhang, Xiaomei 3; Liu, Zhixiong 1; Zhang, Dandan 1; Wang, Jinzi 1; Liu, Di 1,2; Li, Fenglan 1,2; Lu, Hai 1,2
刊名	MOLECULAR BIOLOGY REPORTS
	2012
卷号	39 期号:3 页码:2875-2881
关键词	C-class MADS box gene AGAMOUS Hosta plantaginea Aschers Stamen Gynoecium
ISSN号	0301-4851
DOI	10.1007/s11033-011-1047-0
通讯作者	Wang, Ying
英文摘要	Based on genetic and molecular analyses, the ABC model was proposed to explain the genetic control of floral development. The C-class MADS box gene AGAMOUS (AG) plays crucial roles in Arabidopsis thaliana development through regulation of the organ identity of stamens and gynoecium. The present research reports for the first time the cloning of an AG homologue (HpAG) from Hosta plantaginea Aschers. Phylogenetic analysis shows HpAG is a homologue of AG that is closely related to C-lineage AG homologues from monocot species. Semi-quantitative PCR and real-time PCR analyses show that HpAG is stamen-and gynoecium-specific in expression and has spatial and temporal expression patterns in the reproductive organs of H. plantaginea. The transcriptional activation property of HpAG is also verified by a yeast one-hybrid. Functional analysis is carried out in Arabidopsis by overexpression of HpAG. The homeotic transformations of petals into staminoid organs are observed in 35S::HpAG transgenic plants. All these results show that HpAG1 plays a crucial role in stamen specification and gynoecium development.
学科主题	Biochemistry & Molecular Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000301107800094
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5196]
专题	作物科学研究所_分子生物学系
作者单位	1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol 162, Beijing 100083, Peoples R China 2.Minist Educ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Beijing 100083, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ying,Zhang, Xiaomei,Liu, Zhixiong,et al. Isolation and characterization of an AGAMOUS-like gene from Hosta plantaginea[J]. MOLECULAR BIOLOGY REPORTS,2012,39(3):2875-2881.
APA	Wang, Ying.,Zhang, Xiaomei.,Liu, Zhixiong.,Zhang, Dandan.,Wang, Jinzi.,...&Lu, Hai.(2012).Isolation and characterization of an AGAMOUS-like gene from Hosta plantaginea.MOLECULAR BIOLOGY REPORTS,39(3),2875-2881.
MLA	Wang, Ying,et al."Isolation and characterization of an AGAMOUS-like gene from Hosta plantaginea".MOLECULAR BIOLOGY REPORTS 39.3(2012):2875-2881.