Roles of DICER-LIKE and ARGONAUTE Proteins in TAS-Derived Small Interfering RNA-Triggered DNA Methylation

doi:10.1104/pp.112.200279

	Roles of DICER-LIKE and ARGONAUTE Proteins in TAS-Derived Small Interfering RNA-Triggered DNA Methylation
	Wu, Liang 1,2; Mao, Long 1; Qi, Yijun 2,3
刊名	PLANT PHYSIOLOGY
	2012
卷号	160 期号:2 页码:990-999
ISSN号	0032-0889
DOI	10.1104/pp.112.200279
通讯作者	Wu, Liang
英文摘要	Trans-acting small interfering RNAs (ta-siRNAs; TAS) emerge as a class of plant-specific small RNAs that are initiated from microRNA-mediated cleavage of TAS gene transcripts. It has been revealed that ta-siRNAs are generated by the sequential activities of SUPPRESSOR OF GENE SILENCING3 (SGS3), RNA-DEPENDENT RNA POLYMERASE6 (RDR6), and DICER-LIKE4 (DCL4), and loaded into ARGONAUTE1 (AGO1) proteins to posttranscriptionally regulate several target genes by messenger RNA cleavage in trans. Here, we showed a high cytosine DNA methylation status at ta-siRNA-generating loci in Arabidopsis (Arabidopsis thaliana), which is dependent on RDR6, SGS3, and DNA-DIRECTED RNA POLYMERASE V (PolV). More important, we found that DCL1 is the only DCL protein that is required for TAS3 loci DNA methylation, and all four DCLs exert combinatory functions in the methylation of TAS1 loci, suggesting a previously unknown role for DCL1 in directly processing TAS gene transcripts. Furthermore, we demonstrated that AGO4/6 complexes rather than AGO1 are responsible for TAS siRNA-guided DNA methylation. Based upon these findings, we propose a novel ta-siRNA pathway that acts at both the messenger RNA and chromatin level.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000309523800033
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5126]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Resources & Genet Improvement, Beijing 100081, Peoples R China 2.Natl Inst Biol Sci, Beijing 102206, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Wu, Liang,Mao, Long,Qi, Yijun. Roles of DICER-LIKE and ARGONAUTE Proteins in TAS-Derived Small Interfering RNA-Triggered DNA Methylation[J]. PLANT PHYSIOLOGY,2012,160(2):990-999.
APA	Wu, Liang,Mao, Long,&Qi, Yijun.(2012).Roles of DICER-LIKE and ARGONAUTE Proteins in TAS-Derived Small Interfering RNA-Triggered DNA Methylation.PLANT PHYSIOLOGY,160(2),990-999.
MLA	Wu, Liang,et al."Roles of DICER-LIKE and ARGONAUTE Proteins in TAS-Derived Small Interfering RNA-Triggered DNA Methylation".PLANT PHYSIOLOGY 160.2(2012):990-999.