Isolation and identification of a wheat gene encoding a zinc finger protein (TaZnFP) responsive to abiotic stresses

doi:10.1007/s11738-012-1202-9

	Isolation and identification of a wheat gene encoding a zinc finger protein (TaZnFP) responsive to abiotic stresses
	Min, Dong-Hong 1; Zhao, Yue 1; Huo, Dong-Ying 1; Li, Lian-Cheng 2; Chen, Ming 2; Xu, Zhao-Shi 2; Ma, You-Zhi 2
刊名	ACTA PHYSIOLOGIAE PLANTARUM
	2013
卷号	35 期号:5 页码:1597-1604
关键词	CCCH zinc finger Abiotic stress Transgenic plants Triticum aestivum
ISSN号	0137-5881
DOI	10.1007/s11738-012-1202-9
通讯作者	Min, Dong-Hong
英文摘要	Zinc finger protein is one of the proteins with finger-like domains. CCCH-type zinc finger proteins involved in plant stress tolerance are poorly understood. In this paper, a novel CCCH-type zinc finger protein gene TaZnFP was isolated during analysis of an abiotic stress suppression subtractive hybridization expression library from wheat (Triticum aestivum L.). The TaZnFP gene contains a 1,533 bp open reading frame with no intron and encodes a 55.2 kDa protein with 510 amino acids. Its isoelectric point (pI) is 8.94. The TaZnFP protein contains two typical CCCH-type zinc finger domains (CX9CX5CX3H and CX5CX4CX3H). Transient expression of a 35S::TaZnFP-GFP fusion protein in Arabidopsis mesophyll protoplasts showed that TaZnFP was localized in the cytoplasm. Q-RT-PCR results showed that the TaZnFP transcript was induced by cold, salt (NaCl), drought, and ABA. Overexpression of TaZnFP enhanced drought and salt stress tolerance in transgenic Arabidopsis compared to the control. TaZnFP might be an important regulator responsive to abiotic stresses in plants.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000317969400021
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5043]
专题	作物科学研究所_分子生物学系
作者单位	1.Northwest A&F Univ, Coll Agron, State Key Lab Arid Reg Crop Advers Biol, Yangling 712100, Shanxi, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci,Minist Agr, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticeae Crops, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Min, Dong-Hong,Zhao, Yue,Huo, Dong-Ying,et al. Isolation and identification of a wheat gene encoding a zinc finger protein (TaZnFP) responsive to abiotic stresses[J]. ACTA PHYSIOLOGIAE PLANTARUM,2013,35(5):1597-1604.
APA	Min, Dong-Hong.,Zhao, Yue.,Huo, Dong-Ying.,Li, Lian-Cheng.,Chen, Ming.,...&Ma, You-Zhi.(2013).Isolation and identification of a wheat gene encoding a zinc finger protein (TaZnFP) responsive to abiotic stresses.ACTA PHYSIOLOGIAE PLANTARUM,35(5),1597-1604.
MLA	Min, Dong-Hong,et al."Isolation and identification of a wheat gene encoding a zinc finger protein (TaZnFP) responsive to abiotic stresses".ACTA PHYSIOLOGIAE PLANTARUM 35.5(2013):1597-1604.