BioVector, a flexible system for gene specific-expression in plants

doi:10.1186/1471-2229-13-198

	BioVector, a flexible system for gene specific-expression in plants
	Wang, Xu; Fan, Chengming; Zhang, Xiaomei; Zhu, Jinlong; Fu, Yong-Fu
刊名	BMC PLANT BIOLOGY
	2013
卷号	13 页码:-
关键词	Expression vector Gene specific-expression Functional genome study Gene cloning Restriction enzyme/ligase strategy Gateway DNA recombination
ISSN号	1471-2229
DOI	10.1186/1471-2229-13-198
通讯作者	Wang, Xu
英文摘要	Background: Functional genomic research always needs to assemble different DNA fragments into a binary vector, so as to express genes with different tags from various promoters with different levels. The cloning systems available bear similar disadvantages, such as promoters/tags are fixed on a binary vector, which is generally with low cloning efficiency and limited for cloning sites if a novel promoter/tag is in need. Therefore, it is difficult both to assemble a gene and a promoter together and to modify the vectors in hand. Another disadvantage is that a long spacer from recombination sites, which may be detrimental to the protein function, exists between a gene and a tag. Multiple GATEWAY system only resolves former problem at the expense of very low efficiency and expensive for multiple LR reaction.Results: To improve efficiency and flexibility for constructing expression vectors, we developed a platform, BioVector, by combining classical restriction enzyme/ligase strategy with modern Gateway DNA recombination system. This system included a series of vectors for gene cloning, promoter cloning, and binary vector construction to meet various needs for plant functional genomic study.Conclusion: This BioVector platform makes it easy to construct any vectors to express a target gene from a specific promoter with desired intensity, and it is also waiting to be freely modified by researchers themselves for ongoing demands. This idea can also be transferred to the different fields including animal or yeast study.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000329071700001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4972]
专题	作物科学研究所_分子生物学系
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resource & Genet Improve, MOA Key Lab Soybean Biol Beijing, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xu,Fan, Chengming,Zhang, Xiaomei,et al. BioVector, a flexible system for gene specific-expression in plants[J]. BMC PLANT BIOLOGY,2013,13:-.
APA	Wang, Xu,Fan, Chengming,Zhang, Xiaomei,Zhu, Jinlong,&Fu, Yong-Fu.(2013).BioVector, a flexible system for gene specific-expression in plants.BMC PLANT BIOLOGY,13,-.
MLA	Wang, Xu,et al."BioVector, a flexible system for gene specific-expression in plants".BMC PLANT BIOLOGY 13(2013):-.