Conserved CO-FT regulons contribute to the photoperiod flowering control in soybean

doi:10.1186/1471-2229-14-9

	Conserved CO-FT regulons contribute to the photoperiod flowering control in soybean
	Fan, Chengming 1,2; Hu, Ruibo 3; Zhang, Xiaomei 1; Wang, Xu 1; Zhang, Wenjing 1; Zhang, Qingzhe 1; Ma, Jinhua 1; Fu, Yong-Fu 1
刊名	BMC PLANT BIOLOGY
	2014
卷号	14 页码:-
关键词	CONSTANS FLOWERING LOCUS T Paralog Ortholog Functional divergence Soybean
ISSN号	1471-2229
DOI	10.1186/1471-2229-14-9
通讯作者	Fan, Chengming
英文摘要	Background: CO and FT orthologs, belonging to the BBX and PEBP family, respectively, have important and conserved roles in the photoperiod regulation of flowering time in plants. Soybean genome experienced at least three rounds of whole genome duplications (WGDs), which resulted in multiple copies of about 75% of genes. Subsequent subfunctionalization is the main fate for paralogous gene pairs during the evolutionary process.Results: The phylogenic relationships revealed that CO orthologs were widespread in the plant kingdom while FT orthologs were present only in angiosperms. Twenty-eight CO homologous genes and twenty-four FT homologous genes were gained in the soybean genome. Based on the collinear relationship, the soybean ancestral CO ortholog experienced three WGD events, but only two paralogous gene pairs (GmCOL1/2 and GmCOL5/13) survived in the modern soybean. The paralogous gene pairs, GmCOL1/2 or GmCOL5/13, showed similar expression patterns in pair but different between pairs, indicating that they functionally diverged. GmFTL1 to 7 were derived from the same ancestor prior to the whole genome triplication (WGT) event, and after the Legume WGD event the ancestor diverged into two branches, GmFTL3/5/7 and GmFTL1/2/4/6. GmFTL7 were truncated in the N-terminus compared to other FT-lineage genes, but ubiquitously expressed. Expressions of GmFTL1 to 6 were higher in leaves at the flowering stage than that at the seedling stage. GmFTL3 was expressed at the highest level in all tissues except roots at the seedling stage, and its circadian pattern was different from the other five ones. The transcript of GmFTL6 was highly accumulated in seedling roots. The circadian rhythms of GmCOL5/13 and GmFT1/2/4/5/6 were synchronized in a day, demonstrating the complicate relationship of CO-FT regulons in soybean leaves. Over-expression of GmCOL2 did not rescue the flowering phenotype of the Arabidopsis co mutant. However, ectopic expression of GmCOL5 did rescue the co mutant phenotype. All GmFTL1 to 6 showed flower-promoting activities in Arabidopsis.Conclusions: After three recent rounds of whole genome duplications in the soybean, the paralogous genes of CO-FT regulons showed subfunctionalization through expression divergence. Then, only GmCOL5/13 kept flowering-promoting activities, while GmFTL1 to 6 contributed to flowering control. Additionally, GmCOL5/13 and GmFT1/2/3/4/5/6 showed similar circadian expression profiles. Therefore, our results suggested that GmCOL5/13 and GmFT1/2/3/4/5/6 formed the complicate CO-FT regulons in the photoperiod regulation of flowering time in soybean.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000330073100003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4943]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resource & Genet Improve, MOA Key Lab Soybean Biol Beijing, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China 3.Chinese Acad Sci, Qingdao Inst BioEnergy & BioProc Technol, Shandong Prov Key Lab Energy Genet, CAS Qingdao Inst BioEnergy, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Fan, Chengming,Hu, Ruibo,Zhang, Xiaomei,et al. Conserved CO-FT regulons contribute to the photoperiod flowering control in soybean[J]. BMC PLANT BIOLOGY,2014,14:-.
APA	Fan, Chengming.,Hu, Ruibo.,Zhang, Xiaomei.,Wang, Xu.,Zhang, Wenjing.,...&Fu, Yong-Fu.(2014).Conserved CO-FT regulons contribute to the photoperiod flowering control in soybean.BMC PLANT BIOLOGY,14,-.
MLA	Fan, Chengming,et al."Conserved CO-FT regulons contribute to the photoperiod flowering control in soybean".BMC PLANT BIOLOGY 14(2014):-.