Cloning and Characterization of TaPP2AbB"-alpha, a Member of the PP2A Regulatory Subunit in Wheat

doi:10.1371/journal.pone.0094430

	Cloning and Characterization of TaPP2AbB"-alpha, a Member of the PP2A Regulatory Subunit in Wheat
	Liu, Dan; Li, Ang; Mao, Xinguo; Jing, Ruilian
刊名	PLOS ONE
	2014
卷号	9 期号:4 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0094430
通讯作者	Liu, Dan
英文摘要	Protein phosphatase 2A (PP2A), a major Serine/Threonine protein phosphatase, consists of three subunits; a highly conserved structural subunit A, a catalytic subunit C, and a highly variable regulatory subunit B which determines the substrate specificity. Although the functional mechanism of PP2A in signaling transduction in Arabidopsis is known, their physiological roles in wheat remain to be characterized. In this study, we identified a novel regulatory subunit B, TaPP2AbB"-alpha, in wheat (Triticum aestivum L.). Subcellular localization indicated that TaPP2AbB"-alpha is located in the cell membrane, cytoplasm and nucleus. It interacts with both TaPP2Aa and TaPP2Ac. Expression pattern analyses revealed that TaPP2AbB"-a is strongly expressed in roots, and responds to NaCl, polyethylene glycol (PEG), cold and abscisic acid (ABA) stresses at the transcription level. Transgenic Arabidopsis plants overexpressing TaPP2AbB"-alpha developed more lateral roots, especially when treated with mannitol or NaCl. These results suggest that TaPP2AbB"-alpha, in conjunction with the other two PP2A subunits, is involved in multi-stress response, and positively regulates lateral root development under osmotic stress.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000334159800145
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4903]
专题	作物科学研究所_分子生物学系
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Liu, Dan,Li, Ang,Mao, Xinguo,et al. Cloning and Characterization of TaPP2AbB"-alpha, a Member of the PP2A Regulatory Subunit in Wheat[J]. PLOS ONE,2014,9(4):-.
APA	Liu, Dan,Li, Ang,Mao, Xinguo,&Jing, Ruilian.(2014).Cloning and Characterization of TaPP2AbB"-alpha, a Member of the PP2A Regulatory Subunit in Wheat.PLOS ONE,9(4),-.
MLA	Liu, Dan,et al."Cloning and Characterization of TaPP2AbB"-alpha, a Member of the PP2A Regulatory Subunit in Wheat".PLOS ONE 9.4(2014):-.