Co-treatment with surfactant and sonication significantly improves Agrobacterium-mediated resistant bud formation and transient expression efficiency in soybean

doi:10.1016/S2095-3119(14)60907-2

	Co-treatment with surfactant and sonication significantly improves Agrobacterium-mediated resistant bud formation and transient expression efficiency in soybean
	Guo Bing-fu 1,2; Guo Yong 2; Wang Jun 2,3; Zhang Li-juan 2; Jin Long-guo 2; Hong Hui-long 2; Chang Ru-zheng 2; Qiu Li-juan 2
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2015
卷号	14 期号:7 页码:1242-1250
关键词	soybean genetic transformation sonication surfactant co-treatment
ISSN号	2095-3119
DOI	10.1016/S2095-3119(14)60907-2
通讯作者	Guo Bing-fu
英文摘要	Soybean is a widely planted genetically modified crop around the world. However, it is still one of the most recalcitrant crops for genetic transformation due to the difficulty of regeneration via organogenesis and some factors that affect the transformation efficiency. The percentages of resistant bud formation and transient expression efficiency are important indexes reflecting the regeneration and transformation efficiency of soybean. In this study, the percentages of resistant bud formation and transient expression of beta-glucuronidase (GUS) were compared after treatment with sonication or surfactant and co-treatment with both. The results showed that treatment with either sonication or surfactant increased the percentage of resistant bud formation and transient expression efficiency. The highest percentages were acquired and significantly improved when cotyledon node explants were co-treated with sonication for 2 s and surfactant at 0.02% (v:v) using two different soybean genotypes, Jack and Zhonghuang 10. The improved transformation efficiency of this combination was also evaluated by development of herbicide-tolerant soybeans with transformation efficiency at 2.5-5.7% for different genotypes, which was significantly higher than traditional cotyledonary node method in this study. These results suggested that co-treatment with surfactant and sonication significantly improved the percentages of resistance bud formation, transient expression efficiency and stable transformation efficiency in soybean.
学科主题	Agriculture, Multidisciplinary
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000358188200003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4797]
专题	作物科学研究所_分子生物学系
作者单位	1.Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 3.Yangtze Univ, Coll Agr, Jingzhou 434025, Peoples R China
推荐引用方式 GB/T 7714	Guo Bing-fu,Guo Yong,Wang Jun,et al. Co-treatment with surfactant and sonication significantly improves Agrobacterium-mediated resistant bud formation and transient expression efficiency in soybean[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2015,14(7):1242-1250.
APA	Guo Bing-fu.,Guo Yong.,Wang Jun.,Zhang Li-juan.,Jin Long-guo.,...&Qiu Li-juan.(2015).Co-treatment with surfactant and sonication significantly improves Agrobacterium-mediated resistant bud formation and transient expression efficiency in soybean.JOURNAL OF INTEGRATIVE AGRICULTURE,14(7),1242-1250.
MLA	Guo Bing-fu,et al."Co-treatment with surfactant and sonication significantly improves Agrobacterium-mediated resistant bud formation and transient expression efficiency in soybean".JOURNAL OF INTEGRATIVE AGRICULTURE 14.7(2015):1242-1250.