Ubiquitin-specific protease 24 negatively regulates abscisic acid signalling in Arabidopsis thaliana

doi:10.1111/pce.12628

	Ubiquitin-specific protease 24 negatively regulates abscisic acid signalling in Arabidopsis thaliana
	Zhao, Jinfeng 1; Zhou, Huapeng 2,3; Zhang, Ming 4,5; Gao, Yanan 1; Li, Long 6; Gao, Ying 1; Li, Ming 4; Yang, Yuhong 6; Guo, Yan 2; Li, Xueyong 1
刊名	PLANT CELL AND ENVIRONMENT
	2016
卷号	39 期号:2 页码:427-440
关键词	ABA overly sensitive deubiquitinating enzyme drought sensitivity salt stress stomata protein phosphatase 2C
ISSN号	0140-7791
DOI	10.1111/pce.12628
通讯作者	Zhao, Jinfeng
英文摘要	Abscisic acid (ABA) is an important plant hormone integrating environmental stress and plant growth. Protein ubiquitination and deubiquitination are reversible processes catalysed by E3 ubiquitin ligase and deubiquitinating enzyme, respectively. Lots of E3 ubiquitin ligase and transcriptional factors modified by ubiquitination were reported to modulate ABA signalling. However, no deubiquitinating enzyme has been identified that functions in ABA signalling until now. Here, we isolated an ABA overly sensitive mutant, ubp24, in which the gene encoding ubiquitin-specific protease 24 (UBP24, At4g30890) was disrupted by a T-DNA insertion. The ubp24 mutant was hypersensitive to ABA and salt stress in both postgerminative growth and seedling growth. However, stomata closure in the ubp24 mutant was less sensitive to ABA, and the ubp24 mutant showed drought sensitivity. UBP24 possessed deubiquitinating enzyme activity, and the activity was essential for UBP24 function. Additionally, UBP24 formed homodimer in vivo. UBP24 was genetically upstream of ABI2, and the phosphatase activity of protein phosphatase 2C was decreased in the ubp24 mutant compared with the wild type in the presence of ABA. These results uncover an important regulatory role for the ubiquitin-specific protease in response to ABA and salt stress in plant.
学科主题	Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000370137300016
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4584]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 3.Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China 4.Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China 5.Heilongjiang Acad Agr Sci, Ind Crop Inst, Harbin 150086, Peoples R China 6.Shenyang Agr Univ, Coll Life Sci, Shenyang 110161, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jinfeng,Zhou, Huapeng,Zhang, Ming,et al. Ubiquitin-specific protease 24 negatively regulates abscisic acid signalling in Arabidopsis thaliana[J]. PLANT CELL AND ENVIRONMENT,2016,39(2):427-440.
APA	Zhao, Jinfeng.,Zhou, Huapeng.,Zhang, Ming.,Gao, Yanan.,Li, Long.,...&Li, Xueyong.(2016).Ubiquitin-specific protease 24 negatively regulates abscisic acid signalling in Arabidopsis thaliana.PLANT CELL AND ENVIRONMENT,39(2),427-440.
MLA	Zhao, Jinfeng,et al."Ubiquitin-specific protease 24 negatively regulates abscisic acid signalling in Arabidopsis thaliana".PLANT CELL AND ENVIRONMENT 39.2(2016):427-440.