OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice

doi:10.1016/j.plantsci.2016.05.005

	OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice
	Zhang, Zhe 1; Cheng, Zhi-jun 1; Gan, Lu 1; Zhang, Huan 2; Wu, Fu-qing 1; Lin, Qi-bing 1; Wang, Jiu-lin 1; Wang, Jie 1; Guo, Xiu-ping 1; Zhang, Xin 1
刊名	PLANT SCIENCE
	2016
卷号	249 页码:35-45
关键词	Cuticular wax Hydroxysteroid dehydrogenase Lipid Oil body Oryza sativa
ISSN号	0168-9452
DOI	10.1016/j.plantsci.2016.05.005
通讯作者	Zhang, Zhe
英文摘要	Cuticular wax, a hydrophobic layer on the surface of all aerial plant organs, has essential roles in plant growth and survival under various environments. Here we report a wax-deficient rice mutant oshsd1 with reduced epicuticular wax crystals and thicker cuticle membrane. Quantification of the wax components and fatty acids showed elevated levels of very-long-chain fatty acids (VLCFAs) and accumulation of soluble fatty acids in the leaves of the oshsd1 mutant. We determined the causative gene OsHSD1, a member of the short-chain dehydrogenase reductase family, through map-based cloning. It was ubiquitously expressed and responded to cold stress and exogenous treatments with NaCl or brassinosteroid analogs. Transient expression of OsHSD1-tagged green fluorescent protein revealed that OsHSD1 localized to both oil bodies and endoplasmic reticulum (ER). Dehydrogenase activity assays demonstrated that OsHSD1 was an NAD(+)/NADP(+)-dependent sterol dehydrogenase. Furthermore, OsHSD1 mutation resulted in faster protein degradation, but had no effect on the dehydrogenase activity. Together, our data indicated that OsHSD1 plays a specialized role in cuticle formation and lipid homeostasis, probably by mediating sterol signaling. This work provides new insights into oil-body associated proteins involved in wax and lipid metabolism. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000379104300004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4484]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zhe,Cheng, Zhi-jun,Gan, Lu,et al. OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice[J]. PLANT SCIENCE,2016,249:35-45.
APA	Zhang, Zhe.,Cheng, Zhi-jun.,Gan, Lu.,Zhang, Huan.,Wu, Fu-qing.,...&Wan, Jian-min.(2016).OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice.PLANT SCIENCE,249,35-45.
MLA	Zhang, Zhe,et al."OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice".PLANT SCIENCE 249(2016):35-45.