Characterization of novel LMW-GS genes at Glu-D3 locus on chromosome 1D in Aegilops tauschii

doi:10.1111/j.1601-5223.2008.02046.x

	Characterization of novel LMW-GS genes at Glu-D3 locus on chromosome 1D in Aegilops tauschii
	Zhao, Xianlin 1,2,3; Yang, Yan 1; He, Zhonghu 1,4; Lei, Zhensheng 2; Ma, Wujun 5; Sun, Qixin 3; Xia, Xianchun 1
刊名	HEREDITAS
	2008
卷号	145 期号:5 页码:238-250
ISSN号	0018-0661
DOI	10.1111/j.1601-5223.2008.02046.x
通讯作者	Zhao, Xianlin
英文摘要	The objectives of this study were to clarify the relationship between LMW-GS Glu-D3 gene of Ae. tauschii registered in GenBank and the six Glu-D3 genes including 12 allelic variants of common wheat characterized in our previous studies, and identify novel Glu-D3 genes and haplotypes from Ae. tauschii using gene specific PCR amplification. By searching the NCBI database, 13 LMW-GS genes/pseudogenes of Ae. tauschii were retrieved and classified into five gene families based on their nucleotide similarity with the six Glu-D3 genes of common wheat. Of them, four Ae. tauschii genes, AY585350, AY585354, AY585355 and AY585356 matched GluD3-4, GluD3-5, GluD3-1 and GluD3-2 of common wheat, respectively, and one pseudogene AY585351 matched to GluD3-6, but none of them matched to GluD3-3. In order to identify the Glu-D3 genes from Ae. tauschii corresponding to GluD3-3 and GluD3-6 of common wheat, gene specific primers were developed to amplify 8-18 Ae. tauschii entries. As a result, two novel Glu-D3 genes, designated as GluDt3-3 and GluDt3-6, were identified. GluDt3-3 showed seven allelic variants or haplotypes at the DNA level in eight Ae. tauschii entries, designated as GluDt3-31, GluDt3-32, GluDt3-33, GluDt3-34, GluDt3-35, GluDt3-36 and GluDt3-37, respectively. Two to eight SNPs were found among the seven haplotypes and 1-4 amino acid substitutions among the deduced peptides. Multiple sequence alignments showed that the DNA similarity was 99.6-99.9% among the seven GluDt3-3 haplotypes, and 99.4-99.7% between these haplotypes and those of common wheat GluD3-3 gene. GluDt3-6 presented seven haplotypes in 18 Ae. tauschii entries, designated as GluDt3-61, GluDt3-62, GluDt3-63, GluDt3-64, GluDt3-65, GluDt3-66 and GluDt3-67, respectively. GluDt3-61 from Ae. tauschii entry Ae38 was the only one haplotype with complete coding sequence, and the other six were all pseudogenes. Compared with GluD3-6 gene of common wheat, GluDt3-61 exhibited a 3-bp insertion, a 42-bp deletion and 11 base substitutions, leading to a glutamine insertion in position 52, 14 amino acid deletion in position 84-97 and 10 amino acid mutations in its deduced peptide; GluDt3-62 and GluDt3-63 showed a 6-bp insertion, a 24-bp deletion and 15-21 base substitutions in coding region, of which a nonsense mutation from C to T at position 622 resulted in pseudogenes; GluDt3-64 had five base substitution, including a nonsense mutation at the position 742. GluDt3-65, GluDt3-66 and GluDt3-67 all had a base deletion at position 247, as well as 7-8 base substitutions, which resulted in frameshift mutations in the three haplotypes. The results indicated that Ae. tauschii also contains six Glu-D3 genes and their allelic variants are even richer than those in common wheat.
学科主题	Genetics & Heredity ; GENETICS & HEREDITY
语种	英语
出版者	WILEY-BLACKWELL PUBLISHING, INC
WOS记录号	WOS:000261124200005
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5578]
专题	作物科学研究所_遗传育种系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, CH-100081 Beijing, Peoples R China 2.Henan Acad Agr Sci, Wheat Res Inst, Zhengzhou, Henan, Peoples R China 3.China Agr Univ, Coll Agron & Biotechnol, Beijing 100094, Peoples R China 4.Chinese Acad Agr Sci, China Off, Int Maize & Wheat Improvement Ctr CIMMYT, CH-100081 Beijing, Peoples R China 5.Murdoch Univ Western Australia, State Agr Biotechnol Ctr, Mol Plant Breeding CRC, Dept Agr & Food, Perth, WA, Australia
推荐引用方式 GB/T 7714	Zhao, Xianlin,Yang, Yan,He, Zhonghu,et al. Characterization of novel LMW-GS genes at Glu-D3 locus on chromosome 1D in Aegilops tauschii[J]. HEREDITAS,2008,145(5):238-250.
APA	Zhao, Xianlin.,Yang, Yan.,He, Zhonghu.,Lei, Zhensheng.,Ma, Wujun.,...&Xia, Xianchun.(2008).Characterization of novel LMW-GS genes at Glu-D3 locus on chromosome 1D in Aegilops tauschii.HEREDITAS,145(5),238-250.
MLA	Zhao, Xianlin,et al."Characterization of novel LMW-GS genes at Glu-D3 locus on chromosome 1D in Aegilops tauschii".HEREDITAS 145.5(2008):238-250.