Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing | |
Wu SuoWei1; Xiao Yang2; Zheng Xu1,2; Cai YingFan3; Dolezel, Jaroslav4; Liu BingHua1; Yang Li1; Song MeiFang1,5; Zhou Peng1; Zhou Yang1 | |
刊名 | CHINESE SCIENCE BULLETIN
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2010 | |
卷号 | 55期号:15页码:1463-1468 |
关键词 | Triticum aestivum flow cytogenetics chromosome sorting physical map wheat genome sequencing |
ISSN号 | 1001-6538 |
DOI | 10.1007/s11434-010-3106-7 |
通讯作者 | Wu SuoWei |
英文摘要 | The large genome size (similar to 17000 Mb) and complicated DNA structures of common wheat (Triticum aestivum) hamper its genome sequencing. By means of flow cytometry, systematic investigations on individual chromosome sorting have been carried out to construct chromosome-specific bacterial artificial chromosome (BAC) libraries since the 1980s. Several wheat chromosome-specific BAC libraries, such as chromosome 3B, three D genome chromosomes (1D, 4D and 6D), and the short arm of chromosome 1B, have been developed, and the physical map of chromosome 3B was established in 2008. The same chromosome-based strategy is being employed by the International Wheat Genome Sequencing Consortium (IWGSC) to establish the physical maps of the other 20 common wheat chromosomes (cv. Chinese Spring). Several projects on wheat genome sequencing are currently underway. The availability of new sequencing technologies provides new choices for sequencing of gene space of the wheat genome. The applications of flow-sorted chromosomes in wheat genome studies present some examples to analyze the complex genomes of cereals. |
学科主题 | Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES |
语种 | 英语 |
出版者 | SCIENCE PRESS |
WOS记录号 | WOS:000278704100001 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/5424] ![]() |
专题 | 作物科学研究所_遗传育种系 |
作者单位 | 1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China 3.Chongqing Univ Posts & Telecommun, Coll Bioinformat, Chongqing 400065, Peoples R China 4.Inst Expt Bot, Lab Mol Cytogenet & Cytometry, CZ-77200 Olomouc, Czech Republic 5.Shandong Acad Agr Sci, Inst Vegetable Res, Jinan 250100, Peoples R China |
推荐引用方式 GB/T 7714 | Wu SuoWei,Xiao Yang,Zheng Xu,et al. Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing[J]. CHINESE SCIENCE BULLETIN,2010,55(15):1463-1468. |
APA | Wu SuoWei.,Xiao Yang.,Zheng Xu.,Cai YingFan.,Dolezel, Jaroslav.,...&Yang JianPing.(2010).Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing.CHINESE SCIENCE BULLETIN,55(15),1463-1468. |
MLA | Wu SuoWei,et al."Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing".CHINESE SCIENCE BULLETIN 55.15(2010):1463-1468. |
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