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Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing
Wu SuoWei1; Xiao Yang2; Zheng Xu1,2; Cai YingFan3; Dolezel, Jaroslav4; Liu BingHua1; Yang Li1; Song MeiFang1,5; Zhou Peng1; Zhou Yang1
刊名CHINESE SCIENCE BULLETIN
2010
卷号55期号:15页码:1463-1468
关键词Triticum aestivum flow cytogenetics chromosome sorting physical map wheat genome sequencing
ISSN号1001-6538
DOI10.1007/s11434-010-3106-7
通讯作者Wu SuoWei
英文摘要The large genome size (similar to 17000 Mb) and complicated DNA structures of common wheat (Triticum aestivum) hamper its genome sequencing. By means of flow cytometry, systematic investigations on individual chromosome sorting have been carried out to construct chromosome-specific bacterial artificial chromosome (BAC) libraries since the 1980s. Several wheat chromosome-specific BAC libraries, such as chromosome 3B, three D genome chromosomes (1D, 4D and 6D), and the short arm of chromosome 1B, have been developed, and the physical map of chromosome 3B was established in 2008. The same chromosome-based strategy is being employed by the International Wheat Genome Sequencing Consortium (IWGSC) to establish the physical maps of the other 20 common wheat chromosomes (cv. Chinese Spring). Several projects on wheat genome sequencing are currently underway. The availability of new sequencing technologies provides new choices for sequencing of gene space of the wheat genome. The applications of flow-sorted chromosomes in wheat genome studies present some examples to analyze the complex genomes of cereals.
学科主题Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种英语
出版者SCIENCE PRESS
WOS记录号WOS:000278704100001
内容类型期刊论文
源URL[http://111.203.20.206/handle/2HMLN22E/5424]  
专题作物科学研究所_遗传育种系
作者单位1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
2.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
3.Chongqing Univ Posts & Telecommun, Coll Bioinformat, Chongqing 400065, Peoples R China
4.Inst Expt Bot, Lab Mol Cytogenet & Cytometry, CZ-77200 Olomouc, Czech Republic
5.Shandong Acad Agr Sci, Inst Vegetable Res, Jinan 250100, Peoples R China
推荐引用方式
GB/T 7714
Wu SuoWei,Xiao Yang,Zheng Xu,et al. Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing[J]. CHINESE SCIENCE BULLETIN,2010,55(15):1463-1468.
APA Wu SuoWei.,Xiao Yang.,Zheng Xu.,Cai YingFan.,Dolezel, Jaroslav.,...&Yang JianPing.(2010).Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing.CHINESE SCIENCE BULLETIN,55(15),1463-1468.
MLA Wu SuoWei,et al."Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing".CHINESE SCIENCE BULLETIN 55.15(2010):1463-1468.
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