Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat

doi:10.1186/1471-2229-12-243

	Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat
	Zhang, Xiaofei 1,2; Jin, Hui 1; Zhang, Yan 1; Liu, Dongcheng 2; Li, Genying 3; Xia, Xianchun 1; He, Zhonghu 1,4; Zhang, Aimin 2
刊名	BMC PLANT BIOLOGY
	2012
卷号	12 页码:-
ISSN号	1471-2229
DOI	10.1186/1471-2229-12-243
通讯作者	Zhang, Xiaofei
英文摘要	Background: Low-molecular-weight glutenin subunits (LMW-GS) strongly influence the bread-making quality of bread wheat. These proteins are encoded by a multi-gene family located at the Glu-A3, Glu-B3 and Glu-D3 loci on the short arms of homoeologous group 1 chromosomes, and show high allelic variation. To characterize the genetic and protein compositions of LMW-GS alleles, we investigated 16 Aroona near-isogenic lines (NILs) using SDS-PAGE, 2D-PAGE and the LMW-GS gene marker system. Moreover, the composition of glutenin macro-polymers, dough properties and pan bread quality parameters were determined for functional analysis of LMW-GS alleles in the NILs.Results: Using the LMW-GS gene marker system, 14-20 LMW-GS genes were identified in individual NILs. At the Glu-A3 locus, two m-type and 2-4 i-type genes were identified and their allelic variants showed high polymorphisms in length and nucleotide sequences. The Glu-A3d allele possessed three active genes, the highest number among Glu-A3 alleles. At the Glu-B3 locus, 2-3 m-type and 1-3 s-type genes were identified from individual NILs. Based on the different compositions of s-type genes, Glu-B3 alleles were divided into two groups, one containing Glu-B3a, B3b, B3f and B3g, and the other comprising Glu-B3c, B3d, B3h and B3i. Eight conserved genes were identified among Glu-D3 alleles, except for Glu-D3f. The protein products of the unique active genes in each NIL were detected using protein electrophoresis. Among Glu-3 alleles, the Glu-A3e genotype without i-type LMW-GS performed worst in almost all quality properties. Glu-B3b, B3g and B3i showed better quality parameters than the other Glu-B3 alleles, whereas the Glu-B3c allele containing s-type genes with low expression levels had an inferior effect on bread-making quality. Due to the conserved genes at Glu-D3 locus, Glu-D3 alleles showed no significant differences in effects on all quality parameters.Conclusions: This work provided new insights into the composition and function of 18 LMW-GS alleles in bread wheat. The variation of i-type genes mainly contributed to the high diversity of Glu-A3 alleles, and the differences among Glu-B3 alleles were mainly derived from the high polymorphism of s-type genes. Among LMW-GS alleles, Glu-A3e and Glu-B3c represented inferior alleles for bread-making quality, whereas Glu-A3d, Glu-B3b, Glu-B3g and Glu-B3i were correlated with superior bread-making quality. Glu-D3 alleles played minor roles in determining quality variation in bread wheat. Thus, LMW-GS alleles not only affect dough extensibility but greatly contribute to the dough resistance, glutenin macro-polymers and bread quality.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000314885400001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5103]
专题	作物科学研究所_遗传育种系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Beijing 100081, Peoples R China 2.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Shandong, Peoples R China 4.CAAS, Int Maize & Wheat Improvement Ctr, China Off, CIMMYT, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaofei,Jin, Hui,Zhang, Yan,et al. Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat[J]. BMC PLANT BIOLOGY,2012,12:-.
APA	Zhang, Xiaofei.,Jin, Hui.,Zhang, Yan.,Liu, Dongcheng.,Li, Genying.,...&Zhang, Aimin.(2012).Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat.BMC PLANT BIOLOGY,12,-.
MLA	Zhang, Xiaofei,et al."Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat".BMC PLANT BIOLOGY 12(2012):-.