CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots

doi:10.1371/journal.pone.0136064

	CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots
	Cai, Yupeng 1,2; Chen, Li 1,2; Liu, Xiujie 1,2; Sun, Shi 1; Wu, Cunxiang 1; Jiang, Bingjun 1; Han, Tianfu 1; Hou, Wensheng 1,2
刊名	PLOS ONE
	2015
卷号	10 期号:8 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0136064
通讯作者	Cai, Yupeng
英文摘要	As a new technology for gene editing, the CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system has been rapidly and widely used for genome engineering in various organisms. In the present study, we successfully applied type II CRISPR/Cas9 system to generate and estimate genome editing in the desired target genes in soybean (Glycine max (L.) Merrill.). The single-guide RNA (sgRNA) and Cas9 cassettes were assembled on one vector to improve transformation efficiency, and we designed a sgRNA that targeted a transgene (bar) and six sgRNAs that targeted different sites of two endogenous soybean genes (GmFEI2 and GmSHR). The targeted DNA mutations were detected in soybean hairy roots. The results demonstrated that this customized CRISPR/Cas9 system shared the same efficiency for both endogenous and exogenous genes in soybean hairy roots. We also performed experiments to detect the potential of CRISPR/Cas9 system to simultaneously edit two endogenous soybean genes using only one customized sgRNA. Overall, generating and detecting the CRISPR/Cas9-mediated genome modifications in target genes of soybean hairy roots could rapidly assess the efficiency of each target loci. The target sites with higher efficiencies can be used for regular soybean transformation. Furthermore, this method provides a powerful tool for root-specific functional genomics studies in soybean.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000359666100099
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4676]
专题	作物科学研究所_遗传育种系
作者单位	1.Chinese Acad Agr Sci, Key Lab Soybean Biol Beijing, Inst Crop Sci, Minist Agr, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Ctr Transgen Res Plants, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Cai, Yupeng,Chen, Li,Liu, Xiujie,et al. CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots[J]. PLOS ONE,2015,10(8):-.
APA	Cai, Yupeng.,Chen, Li.,Liu, Xiujie.,Sun, Shi.,Wu, Cunxiang.,...&Hou, Wensheng.(2015).CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots.PLOS ONE,10(8),-.
MLA	Cai, Yupeng,et al."CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots".PLOS ONE 10.8(2015):-.