Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits

doi:10.1007/s10722-010-9561-9

	Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits
	Guo, Xiaomin; Guo, Junxian; Li, Xiuquan; Yang, Xinming; Li, Lihui
刊名	GENETIC RESOURCES AND CROP EVOLUTION
	2010
卷号	57 期号:8 页码:1217-1225
关键词	Chinese wheat landrace Dough properties Glu-D1 HMW-GS ORFs Triticum aestivum
ISSN号	0925-9864
DOI	10.1007/s10722-010-9561-9
通讯作者	Guo, Xiaomin
英文摘要	The high molecular weight glutenin subunits (HMW-GS) can be used for wheat quality improvement. Two novel alleles (designated 1Dx1.5* and 1Dy12.2, respectively) at the Glu-D1 locus were identified in the Chinese wheat landrace variety 'Jiuquanjinbaoyin' by comparison of subunit mobility with that previously identified in several standard hexaploid wheats. The 1Dx1.5 and 1Dy12.2* genes were isolated using the allele-specific PCR primers and the complete open reading frames (ORFs) were obtained. Allele 1Dx1.5* consists of 2487 bp encoding a mature protein of 827 amino acid residues, whereas 1Dy12.2* consists 1980 bp encoding 658 residues. Comparisons of amino acid sequences analysis showed that 1Dx1.5* had higher similarity with the HMW-GS isolated from the wheat related species (Aegilops tauchi Coss.) than from the bread wheat varieties (Triticum aestivum L.). The 1Dy12.2* amino acid sequence showed a generally similar to the 1Dy12* isolated from Chinese endemic wheats. Meanwhile, the dough properties of the lines expressing (null, 7+8, 1.5+10), (null, 7+8, 2+12.2) and (null, 7+8, 2+12), respectively, were measured by a Mixograph, which demonstrates that the alleles 1.5+10 can be considered as having positive impact on dough strength when compared with the alleles 2+12. In addition, the subunits 2+12.2 also showed a greater impact on dough strength than 2+12.
学科主题	Agronomy ; Plant Sciences ; AGRONOMY ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000284777400010
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5381]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Guo, Xiaomin,Guo, Junxian,Li, Xiuquan,et al. Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits[J]. GENETIC RESOURCES AND CROP EVOLUTION,2010,57(8):1217-1225.
APA	Guo, Xiaomin,Guo, Junxian,Li, Xiuquan,Yang, Xinming,&Li, Lihui.(2010).Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits.GENETIC RESOURCES AND CROP EVOLUTION,57(8),1217-1225.
MLA	Guo, Xiaomin,et al."Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits".GENETIC RESOURCES AND CROP EVOLUTION 57.8(2010):1217-1225.