Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps10 in a Chinese Soybean Cultivar

doi:10.1371/journal.pone.0069799

	Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps10 in a Chinese Soybean Cultivar
	Zhang, Jiqing; Xia, Changjian; Duan, Canxing; Sun, Suli; Wang, Xiaoming; Wu, Xiaofei; Zhu, Zhendong
刊名	PLOS ONE
	2013
卷号	8 期号:7 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0069799
通讯作者	Zhang, Jiqing
英文摘要	Resistance to Phytophthora sojae isolate PsMC1 was evaluated in 102 F-2:3 families derived from a cross between the resistant soybean cultivar Wandou 15 and the susceptible cultivar Williams and genotyped using simple sequence repeat (SSR) markers. The segregation ratio of resistant, segregating, and susceptible phenotypes in the population suggested that the resistance in Wandou 15 was dominant and monogenic. Twenty-six polymorphic SSR markers were identified on soybean chromosome 17 (Molecular linkage group D2; MLG D2), which were linked to the resistance gene based on bulked segregation analysis (BSA). Markers Sattwd15-24/25 and Sattwd15-47 flanked the resistance gene at a distance of 0.5 cM and 0.8 cM, respectively. Two cosegregating markers, Sattwd15-28 and Sattwd15-32, were also screened in this region. This is the first Rps resistance gene mapped on chromosome 17, which is designated as Rps10. Eight putative genes were found in the mapped region between markers Sattwd15-24/25 and Sattwd15-47. Among them, two candidate genes encoding serine/threonine (Ser/Thr) protein kinases in Wandou 15 and Williams were identified and sequenced. And the differences in genomic sequence and the putative amino acid sequence, respectively, were identified within each candidate gene between Wandou 15 and Williams. This novel gene Rps10 and the linked markers should be useful in developing soybean cultivars with durable resistance to P. sojae.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000322433300067
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5025]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, MOA Key Lab Soybean Biol Beijing, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jiqing,Xia, Changjian,Duan, Canxing,et al. Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps10 in a Chinese Soybean Cultivar[J]. PLOS ONE,2013,8(7):-.
APA	Zhang, Jiqing.,Xia, Changjian.,Duan, Canxing.,Sun, Suli.,Wang, Xiaoming.,...&Zhu, Zhendong.(2013).Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps10 in a Chinese Soybean Cultivar.PLOS ONE,8(7),-.
MLA	Zhang, Jiqing,et al."Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps10 in a Chinese Soybean Cultivar".PLOS ONE 8.7(2013):-.