Gene mapping and functional analysis of the novel leaf color gene SiYGL1 in foxtail millet [Setaria italica (L.) P. Beauv]

doi:10.1111/ppl.12405

	Gene mapping and functional analysis of the novel leaf color gene SiYGL1 in foxtail millet [Setaria italica (L.) P. Beauv]
	Li, Wen 1,2; Tang, Sha 1; Zhang, Shuo 1; Shan, Jianguo 1,2; Tang, Chanjuan 1; Chen, Qiannan 1; Jia, Guanqing 1; Han, Yuanhuai 1,2; Zhi, Hui 1; Diao, Xianmin 1
刊名	PHYSIOLOGIA PLANTARUM
	2016
卷号	157 期号:1 页码:24-37
ISSN号	0031-9317
DOI	10.1111/ppl.12405
通讯作者	Li, Wen
英文摘要	Setaria italica and its wild ancestor Setaria viridis are emerging as model systems for genetics and functional genomics research. However, few systematic gene mapping or functional analyses have been reported in these promising C4 models. We herein isolated the yellow-green leaf mutant (siygl1) in S. italica using forward genetics approaches. Map-based cloning revealed that SiYGL1, which is a recessive nuclear gene encoding a magnesium-chelatase D subunit (CHLD), is responsible for the mutant phenotype. A single Phe to Leu amino acid change occurring near the ATPase-conserved domain resulted in decreased chlorophyll (Chl) accumulation and modified chloroplast ultrastructure. However, the mutation enhanced the light-use efficiency of the siygl1 mutant, suggesting that the mutated CHLD protein does not completely lose its original activity, but instead, gains novel features. A transcriptional analysis of Chl a oxygenase revealed that there is a strong negative feedback control of Chl b biosynthesis in S. italica. The SiYGL1mRNA was expressed in all examined tissues, with higher expression observed in the leaves. Comparison of gene expression profiles in wild-type and siygl1 mutant plants indicated that SiYGL1 regulates a subset of genes involved in photosynthesis (rbcL and LHCB1), thylakoid development (DEG2) and chloroplast signaling (SRP54CP). These results provide information regarding the mutant phenotype at the transcriptional level. This study demonstrated that the genetic material of a Setaria species could be ideal for gene discovery investigations using forward genetics approaches and may help to explain the molecular mechanisms associated with leaf color variation.
学科主题	Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000374668700003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4532]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.Shanxi Agr Univ, Coll Agron, Taigu 030801, Shanxi, Peoples R China
推荐引用方式 GB/T 7714	Li, Wen,Tang, Sha,Zhang, Shuo,et al. Gene mapping and functional analysis of the novel leaf color gene SiYGL1 in foxtail millet [Setaria italica (L.) P. Beauv][J]. PHYSIOLOGIA PLANTARUM,2016,157(1):24-37.
APA	Li, Wen.,Tang, Sha.,Zhang, Shuo.,Shan, Jianguo.,Tang, Chanjuan.,...&Diao, Xianmin.(2016).Gene mapping and functional analysis of the novel leaf color gene SiYGL1 in foxtail millet [Setaria italica (L.) P. Beauv].PHYSIOLOGIA PLANTARUM,157(1),24-37.
MLA	Li, Wen,et al."Gene mapping and functional analysis of the novel leaf color gene SiYGL1 in foxtail millet [Setaria italica (L.) P. Beauv]".PHYSIOLOGIA PLANTARUM 157.1(2016):24-37.