Genetic transformation of exserohilum turcicum mediated by agrobacterium tumefaciens

CORC > 中国农业科学院 > 作物科学研究所

	Genetic transformation of exserohilum turcicum mediated by agrobacterium tumefaciens
	Zhou Yongli 1; TANAKA Chihiro 2; TSUDA Mitusya 2
刊名	菌物系统
	2003
卷号	22 期号:3 页码:374-379
关键词	T-DNA integration hygromycin B mycelium
ISSN号	1007-3515
其他题名	农杆菌介导的玉米大斑病菌转化
英文摘要	A transformation technique for Exserohilum turcicum mediated by Agrobacterium tumefaciens using vegetative mycelium as recipient was developed and procedure for the enhancement of transformation efficiency was optimized. The binary vector used in transformation was pUR5750, which contains selection marker gene conferring hygromycin-resistance under control of the Aspergillus nidulans gpd promoter and trpC terminator between the left border and right border repeats of the binary vector pB!N19. PCR analysis showed that T-DNA was integrated into the genome of E. turcicum.
学科主题	植物学
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/106443]
专题	作物科学研究所
作者单位	1.Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Genetic and Breeding of Ministry of Agriculture, 北京 2.Faculty of Agriculture, Kyoto University, Japan, Kyoto, 606-8502
推荐引用方式 GB/T 7714	Zhou Yongli,TANAKA Chihiro,TSUDA Mitusya. Genetic transformation of exserohilum turcicum mediated by agrobacterium tumefaciens[J]. 菌物系统,2003,22(3):374-379.
APA	Zhou Yongli,TANAKA Chihiro,&TSUDA Mitusya.(2003).Genetic transformation of exserohilum turcicum mediated by agrobacterium tumefaciens.菌物系统,22(3),374-379.
MLA	Zhou Yongli,et al."Genetic transformation of exserohilum turcicum mediated by agrobacterium tumefaciens".菌物系统 22.3(2003):374-379.