Identification and Preliminary Analysis of Several  Centromere-associated Bacterial Artificial  Chromosome Clones from a Diploid  Wheat Library

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	Identification and Preliminary Analysis of Several Centromere-associated Bacterial Artificial Chromosome Clones from a Diploid Wheat Library
	Zhao Liu; Wei Yue; Yushen Dong; Xueyong Zhang
刊名	Journal of Integrative Plant Biology
	2006
卷号	48 期号:3 页码:348-358
关键词	centromere fluorescence in situ hybridization （FISH） repeat Southern hybridization Triticum boeoticum.
ISSN号	1672-9072
英文摘要	Although the centromeres of some plants have been investlgated prevlously, our knowledge of the wheat centromere Is still very llmlted. To understand the structure and functlon of the wheat centromere, we used two centromeric repeats （RCS1 and CCS1-5ab） to obtain some centromere-assoclated bacterial artificial chromosome （BAC） clones in 32 RCS1-related BAC clones that had been screened out from a diploid wheat （Triticum boeoticum Boiss.; 2n=2x=14） BAC library. Southern hybridization results indicated that, of the 32 candidates, there were 28 RCS1-positive clones. Based on gel blot patterns, the frequency of RCS1 was approximately one copy every 69.4 kb in these 28 RCS1-positive BAC clones. More bands were detected when the same filter was probed with CCS1-5ab. Furthermore, the CCS1 bands covered all the bands detected by RCS1, which suggests that some CCS1 repeats were distributed together with RCS1. The frequency of CCS1 families was once every 35.8 kb, nearly twice that of RCS1. Fluorescence in situ hybridization （FISH） analysis Indicated that the five BAC clones containing RCS1 and CCS1 sequences all detected signals at the centromerlc regions in hexaplold wheat, but the signal intensities on the A-genome chromosomes were stronger than those on the B- and/or Dgenome chromosomes. The FISH analysis among nine Triticeae cereals indicated that there were A-genomespecific （or rich） sequences dispersing on chromosome arms in the BAC clone TbBACS. In addition, at the interphase cells, the centromeres of diploid species usually clustered at one pole and formed a ring-like allocation In the period before metaphase.
学科主题	农作物
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/106229]
专题	作物科学研究所
作者单位	Institute of Crop Sciences，Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Germplasm and Biotechnology, Beijing, 100081
推荐引用方式 GB/T 7714	Zhao Liu,Wei Yue,Yushen Dong,et al. Identification and Preliminary Analysis of Several Centromere-associated Bacterial Artificial Chromosome Clones from a Diploid Wheat Library[J]. Journal of Integrative Plant Biology,2006,48(3):348-358.
APA	Zhao Liu,Wei Yue,Yushen Dong,&Xueyong Zhang.(2006).Identification and Preliminary Analysis of Several Centromere-associated Bacterial Artificial Chromosome Clones from a Diploid Wheat Library.Journal of Integrative Plant Biology,48(3),348-358.
MLA	Zhao Liu,et al."Identification and Preliminary Analysis of Several Centromere-associated Bacterial Artificial Chromosome Clones from a Diploid Wheat Library".Journal of Integrative Plant Biology 48.3(2006):348-358.