Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat

doi:10.1007/s00122-007-0539-8

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	Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat
	He, X. Y.; He, Z. H.; Zhang, L. P.; Sun, D. J.; Morris, C. F.; Fuerst, E. P.; Xia, X. C.
刊名	THEORETICAL AND APPLIED GENETICS
	2007
卷号	115 期号:1 页码:47-58
ISSN号	0040-5752
DOI	10.1007/s00122-007-0539-8
通讯作者	He, X. Y.
英文摘要	Polyphenol oxidase (PPO) activity is highly related to the undesirable browning of wheat-based end products, especially Asian noodles. Characterization of PPO genes and the development of their functional markers are of great importance for marker-assisted selection in wheat breeding. In the present study, complete genomic DNA sequences of two PPO genes, one each located on chromosomes 2A and 2D and their allelic variants were characterized by means of in silico cloning and experimental validation. Sequences were aligned at both DNA and protein levels. Two haplotypes on chromosome 2D showed 95.2% sequence identity at the DNA level, indicating much more sequence diversity than those on chromosome 2A with 99.6% sequence identity. Both of the PPO genes on chromosomes 2A and 2D contain an open reading frame (ORF) of 1,731 bp, encoding a PPO precursor peptide of 577 amino acids with a predicted molecular mass of similar to 64 kD. Two complementary dominant STS markers, PPO16 and PPO29, were developed based on the PPO gene haplotypes located on chromosome 2D; they amplify a 713-bp fragment in cultivars with low PPO activity and a 490-bp fragment in those with high PPO activity, respectively. The two markers were mapped on chromosome 2DL using a doubled haploid population derived from the cross Zhongyou 9507/CA9632, and a set of nullisomic-tetrasomic lines and ditelosomic line 2DS of Chinese Spring. QTL analysis indicated that the PPO gene co-segregated with the two STS markers and was closely linked to SSR marker Xwmc41 on chromosome 2DL, explaining from 9.6 to 24.4% of the phenotypic variance for PPO activity across three environments. In order to simultaneously detect PPO loci on chromosomes 2A and 2D, a multiplexed marker combination PPO33/PPO16 was developed and yielded distinguishable DNA patterns in a number of cultivars. The STS marker PPO33 for the PPO gene on chromosome 2A was developed from the same gene sequences as PPO18 that we reported previously, and can amplify a 481-bp and a 290-bp fragment from cultivars with low and high PPO activity, respectively. A total of 217 Chinese wheat cultivars and advanced lines were used to validate the association between the polymorphic fragments and grain PPO activity. The results showed that the marker combination PPO33/PPO16 is efficient and reliable for evaluating PPO activity and can be used in wheat breeding programs aimed for noodle and other end product quality improvement.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000246739800006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5670]
专题	作物科学研究所
作者单位	(1)Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China;(2)Chinese Acad Agr Sci, CIMMYT, Int Maize & Wheat Improvement Ctr, China Off, Beijing 100081, Peoples R China;(3)Beijing Acad Agr & Forestry Sci, Beijing Engn & Tech Res Ctr Hybrid Wheat, Beijing 100097, Peoples R China;(4)NW Sci Tech Univ Agr & Forestry, Coll Agron, Yangling 712100, Shaanxi, Peoples R China;(5)Washington State Univ, USDA ARS, Western Wheat Qual Lab, Pullman, WA 99164 USA;(6)Washington State Univ, Western Wheat Qual Lab, Dept Crop & Soil Sci, Pullman, WA 99164 USA
推荐引用方式 GB/T 7714	He, X. Y.,He, Z. H.,Zhang, L. P.,et al. Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat[J]. THEORETICAL AND APPLIED GENETICS,2007,115(1):47-58.
APA	He, X. Y..,He, Z. H..,Zhang, L. P..,Sun, D. J..,Morris, C. F..,...&Xia, X. C..(2007).Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat.THEORETICAL AND APPLIED GENETICS,115(1),47-58.
MLA	He, X. Y.,et al."Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat".THEORETICAL AND APPLIED GENETICS 115.1(2007):47-58.