Development of two multiplex PCR assays targeting improvement of bread-making and noodle qualities in common wheat

doi:10.1111/j.1439-0523.2007.01442.x

CORC > 中国农业科学院 > 作物科学研究所

	Development of two multiplex PCR assays targeting improvement of bread-making and noodle qualities in common wheat
	Zhang, X. K.1,2; Liu, L.1; He, Z. H.1,3; Sun, D. J.1; He, X. Y.1; Xu, Z. H.1; Zhang, P. P.1; Chen, F.1; Xia, X. C.1
刊名	PLANT BREEDING
	2008
卷号	127 期号:2 页码:109-115
关键词	Triticum aestivum L. quality multiplex PCR high molecular weight glutenin subunit HMW-GS low molecular weight glutenin subunit LMW-GS Wx-B1 Pinb-D1b PPO activity 1B.1R translocation marker-assisted selection MAS
ISSN号	0179-9541
DOI	10.1111/j.1439-0523.2007.01442.x
通讯作者	Zhang, X. K.
英文摘要	Wheat quality properties are genetically determined by the compositions of high and low molecular weight glutenin subunits, grain hardness, polyphenol oxidase (PPO) activity and starch viscosity. Two multiplex PCR assays were developed and validated using 70 cultivars and advanced lines from Chinese autumn-sown wheat regions. Multiplex PCR I includes molecular markers for genes/loci omega-secalin, Glu-B1-2a (By8), Glu-D1-1d (Dx5), Glu-A3d, Glu-B3 (for non-1B.1R type) and Pinb-D1b targeting improved gluten parameters and pan bread quality. Multiplex PCR II comprises markers for genes/loci Ppo-A1, Ppo-D1 and Wx-B1b targeting improved noodle quality. The results were consistent with those achieved by SDS-PAGE and RP-HPLC, indicating that the two multiplex assays were highly effective, with good repeatability and low costs enabling their use in wheat breeding programmes. In total, nine alleles (subunits) at locus Glu-B1, four at Glu-D1 and five at Glu-A3 locus were identified, and the alleles (Subunits) Glu-B1b (7 + 8), Glu-B1c (7 + 9), Glu-D1a (2 + 12), Glu-D1d (5 + 10), Glu-A3a, Glu-A3c and Glu-A3d were most frequently present in the cultivars and lines tested. The 1B.1R translocation was present in 28 (40.0%) lines, whereas the Wx-B1 null allele for better noodle quality was present in only seven (10.0%) cultivars and advanced lines, and 37 (52.9%) lines had Pinb-D1b associated with hard grains. The allele Ppo-A1b on chromosome 2AL associated with lower PPO activity was present in 38 (54.3%) genotypes, whereas the less effective allele Ppo-D1a on chromosome 2DL, also associated with low PPO activity was present in 45 (64.3%) of genotypes. These two multiplex PCR assays should be effective in marker assisted selection targeting improved pan bread-making and noodle qualities.
学科主题	Agronomy ; Biotechnology & Applied Microbiology ; Plant Sciences ; AGRONOMY ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; PLANT SCIENCES
语种	英语
出版者	BLACKWELL PUBLISHING
WOS记录号	WOS:000254703200001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5614]
专题	作物科学研究所
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.NW A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China 3.CAAS, CIMMYT China Off, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang, X. K.,Liu, L.,He, Z. H.,et al. Development of two multiplex PCR assays targeting improvement of bread-making and noodle qualities in common wheat[J]. PLANT BREEDING,2008,127(2):109-115.
APA	Zhang, X. K..,Liu, L..,He, Z. H..,Sun, D. J..,He, X. Y..,...&Xia, X. C..(2008).Development of two multiplex PCR assays targeting improvement of bread-making and noodle qualities in common wheat.PLANT BREEDING,127(2),109-115.
MLA	Zhang, X. K.,et al."Development of two multiplex PCR assays targeting improvement of bread-making and noodle qualities in common wheat".PLANT BREEDING 127.2(2008):109-115.