Characterization of the TaAIDFa gene encoding a CRT/DRE-binding factor responsive to drought, high-salt, and cold stress in wheat

doi:10.1007/s00438-008-0382-x

CORC > 中国农业科学院 > 作物科学研究所

	Characterization of the TaAIDFa gene encoding a CRT/DRE-binding factor responsive to drought, high-salt, and cold stress in wheat
	Xu, Zhao-Shi 2; Ni, Zhi-Yong 2; Liu, Li 2; Nie, Li-Na 2; Li, Lian-Cheng 2; Chen, Ming 2; Ma, You-Zhi 1,2
刊名	MOLECULAR GENETICS AND GENOMICS
	2008
卷号	280 期号:6 页码:497-508
关键词	Abiotic stress DBF Gene expression Promoter Osmotic stress tolerance Wheat
ISSN号	1617-4615
DOI	10.1007/s00438-008-0382-x
通讯作者	Xu, Zhao-Shi
英文摘要	Dehydration responsive element-binding factors (DBFs) belong to the AP2/ERF superfamily and play vital regulatory roles in abiotic stress responses in plants. In this study, we isolated three novel homologs of the DBF gene family in wheat (Triticum aestivum L.) by screening a drought-induced cDNA library and designated them as TaAIDFs (T. aestivum abiotic stress-induced DBFs). Compared to TaAIDFb and TaAIDFc, TaAIDFa lacks a short Ser/Thr-rich region, a putative phosphorylation site, following the AP2/ERF domain. The TaAIDFa gene, located on chromosome 3BS, is interrupted by a single intron at the 17th Arg (R) in the N-terminal domain. The N-terminal region of the TaAIDFa protein modulates nuclear localization. The TaAIDFa protein is capable of binding to CRT/DRE elements in vitro and in vivo, and of trans-activating reporter gene expression in yeast cells. The TaAIDFa promoter, with various stress-related cis-acting elements, drives expression of the GUS reporter gene in wheat calli under stress conditions. This was further confirmed by responses of TaAIDFa transcripts to drought, salinity, low-temperature, and exogenous ABA. Furthermore, overexpression of TaAIDFa activated CRT/DRE-containing genes under normal growth conditions, and improved drought and osmotic stress tolerances in transgenic Arabidopsis plants. These results suggested that TaAIDFa encodes a CRT/DRE element-binding factor that might be involved in multiple abiotic stress signal transduction pathways.
学科主题	Biochemistry & Molecular Biology ; Genetics & Heredity ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; GENETICS & HEREDITY
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000260960400003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5558]
专题	作物科学研究所
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.Minist Agr, Key Lab Crop Genet & Breeding, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Xu, Zhao-Shi,Ni, Zhi-Yong,Liu, Li,et al. Characterization of the TaAIDFa gene encoding a CRT/DRE-binding factor responsive to drought, high-salt, and cold stress in wheat[J]. MOLECULAR GENETICS AND GENOMICS,2008,280(6):497-508.
APA	Xu, Zhao-Shi.,Ni, Zhi-Yong.,Liu, Li.,Nie, Li-Na.,Li, Lian-Cheng.,...&Ma, You-Zhi.(2008).Characterization of the TaAIDFa gene encoding a CRT/DRE-binding factor responsive to drought, high-salt, and cold stress in wheat.MOLECULAR GENETICS AND GENOMICS,280(6),497-508.
MLA	Xu, Zhao-Shi,et al."Characterization of the TaAIDFa gene encoding a CRT/DRE-binding factor responsive to drought, high-salt, and cold stress in wheat".MOLECULAR GENETICS AND GENOMICS 280.6(2008):497-508.