Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean

doi:10.1007/s11032-009-9272-0

CORC > 中国农业科学院 > 作物科学研究所

	Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean
	Li, Ying-Hui 1; Zhang, Chen 1; Gao, Zhong-Shan 2; Smulders, Marinus Johannes Maria 3; Ma, Zulu 1,4; Liu, Zhang-Xiong 1; Nan, Hai-Yang 1; Chang, Ru-Zhen 1; Qiu, Li-Juan 1
刊名	MOLECULAR BREEDING
	2009
卷号	24 期号:1 页码:63-76
关键词	Haplotype SCN SNP marker Soybean
ISSN号	1380-3743
DOI	10.1007/s11032-009-9272-0
通讯作者	Li, Ying-Hui
英文摘要	Soybean cyst nematode (SCN; Heterodera glycines Ichinohe) is one of the most destructive pests in the cultivation of soybean (Glycine max (L.) Merr.) worldwide. Markers based on the SCN resistance gene will enable efficient marker-assisted selection (MAS). We sequenced the candidate gene rhg1 in six resistant and two susceptible soybean genotypes and identified 37 SNPs (single nucleotide polymorphisms) among the sequences, of which 11 were in the coding region. Seven of these 11 SNPs led to changes in the amino acid sequence of the gene. The amino acid sequence we obtained differs from the previously published one by a stretch of 26-27 amino acids. Six codominant allele-specific SNP markers based on agarose gel detection were developed and tested in 70 genotypes, among which occurred only nine different haplotypes. Two neutrality tests (Tajima's D and Fu and Li's F) were significant for the six SNP loci in the 70 genotypes, which is consistent with intensive directional selection. A strong LD pattern was detected among five SNPs except 2868T > C. Two SNPs (689C > A and 757C > T) formed one haplotype (689C-757C) that was perfectly associated with SCN resistance. The new allele-specific PCR markers located in the alleged sequence of the rhg1 candidate gene, combined with the microsatellite marker BACR-Satt309, will significantly improve the efficiency of MAS during the development of SCN-resistant cultivars.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000267785700006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5504]
专题	作物科学研究所
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Germplasm & Biotechnol MOA, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Zhejiang Univ, Dept Hort, State Agr Minist, Lab Hort Plant Growth Dev & Qual Improvement, Hangzhou 310029, Zhejiang, Peoples R China 3.Wageningen UR, NL-6700 AA Wageningen, Netherlands 4.BengBu Med Coll, Bengbu 233000, Peoples R China
推荐引用方式 GB/T 7714	Li, Ying-Hui,Zhang, Chen,Gao, Zhong-Shan,et al. Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean[J]. MOLECULAR BREEDING,2009,24(1):63-76.
APA	Li, Ying-Hui.,Zhang, Chen.,Gao, Zhong-Shan.,Smulders, Marinus Johannes Maria.,Ma, Zulu.,...&Qiu, Li-Juan.(2009).Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean.MOLECULAR BREEDING,24(1),63-76.
MLA	Li, Ying-Hui,et al."Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean".MOLECULAR BREEDING 24.1(2009):63-76.