Cloning, expression and functional analysis of HMW glutenin subunit 1By8 gene from Italy pasta wheat (Triticum turgidum L. ssp durum)

doi:10.1016/j.jcs.2009.08.004

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	Cloning, expression and functional analysis of HMW glutenin subunit 1By8 gene from Italy pasta wheat (Triticum turgidum L. ssp durum)
	Yan, Y. M.1; Jiang, Y.1; An, X. L.1; Pei, Y. H.1; Li, X. H.1; Zhang, Y. Z.1; Wang, A. L.1,5,6; He, Z.2,3; Xia, X.2,3; Bekes, F.4
刊名	JOURNAL OF CEREAL SCIENCE
	2009
卷号	50 期号:3 页码:398-406
关键词	High molecular weight glutenin By8 gene Bacterial expression Gene effects
ISSN号	0733-5210
DOI	10.1016/j.jcs.2009.08.004
通讯作者	Yan, Y. M.
英文摘要	Cloning and functional analysis of high molecular weight wheat glutenin subunit (HMW-GS) 1By8 from Italy durum cultivar Simeto was carried out in this study. All HMW-GS from Simeto were separated and characterized by appropriate electrophoresis methods, reversed-phased high performance liquid chromatography (RP-HPLC) and mass spectrometry (MS). The complete gene encoding 1By8 subunit was amplified by allele-specific PCR primers, including an upstream sequence of 857 bp and an open reading frame (ORF) of 2166 bp encoding a mature protein of 720 amino acid residues. The promoter sequence, containing -300 element (cereal glutenin gene control element) and enhancer was highly conserved among HMW-GS genes. Comparison with the sequence of subunit 1By9 from bread wheat demonstrated 99% identity with the main difference being that the 1By8 subunit possesses an additional insertion of 15 amino acid residues (QYPASQQQPA QGQQG) at position 342 and two residue substitutions at position 78 (leucine/proline) and 442 (arginine/glutamine). The molecular weight differences between MALDI-TOF-MS and deduced amino acid sequence of the coding gene revealed the possibility of some kinds of post-translational modifications present in 1By8 subunit. The protein subunit expressed in Escherichia coli showed a very similar mobility to the endogenous 1By8 of Simeto on SDS-PAGE. The function of the isolated protein on wheat processing quality was determined by 10 g Mixgraph analysis. Results demonstrated that addition of y-type HMW glutenin subunits into the base flour had significant positive effects on main mixing parameters and significant difference in effects were observed among different y-type subunits. (C) 2009 Elsevier Ltd. All rights reserved.
学科主题	Food Science & Technology ; FOOD SCIENCE & TECHNOLOGY
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000273084500017
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5477]
专题	作物科学研究所
作者单位	1.Capital Normal Univ, Coll Life Sci, Key Lab Genet & Biotechnol, Beijing 100037, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Beijing 100081, Peoples R China 3.Chinese Acad Agr Sci, CIMMYT, China Off, Beijing 100081, Peoples R China 4.CSIRO Plant Ind, Canberra, ACT, Australia 5.Murdoch Univ, Western Australian Dept Agr & Food, Ctr Comparat Genom, Perth, WA 6150, Australia 6.Murdoch Univ, State Agr Biotechnol Ctr, Perth, WA 6150, Australia
推荐引用方式 GB/T 7714	Yan, Y. M.,Jiang, Y.,An, X. L.,et al. Cloning, expression and functional analysis of HMW glutenin subunit 1By8 gene from Italy pasta wheat (Triticum turgidum L. ssp durum)[J]. JOURNAL OF CEREAL SCIENCE,2009,50(3):398-406.
APA	Yan, Y. M..,Jiang, Y..,An, X. L..,Pei, Y. H..,Li, X. H..,...&Ma, W..(2009).Cloning, expression and functional analysis of HMW glutenin subunit 1By8 gene from Italy pasta wheat (Triticum turgidum L. ssp durum).JOURNAL OF CEREAL SCIENCE,50(3),398-406.
MLA	Yan, Y. M.,et al."Cloning, expression and functional analysis of HMW glutenin subunit 1By8 gene from Italy pasta wheat (Triticum turgidum L. ssp durum)".JOURNAL OF CEREAL SCIENCE 50.3(2009):398-406.