Isolation and characterization of a gene encoding a polyethylene glycol-induced cysteine protease in common wheat

doi:10.1007/s12038-010-0043-1

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	Isolation and characterization of a gene encoding a polyethylene glycol-induced cysteine protease in common wheat
	Zang, Qing-Wei 1; Wang, Cai-Xiang 1; Li, Xu-Yan 1; Guo, Zhi-Ai 1; Jing, Rui-Lian 1; Zhao, Jun 2; Chang, Xiao-Ping 1
刊名	JOURNAL OF BIOSCIENCES
	2010
卷号	35 期号:3 页码:379-388
关键词	Cysteine protease TaCP water deficit tolerance gene expression common wheat
ISSN号	0250-5991
DOI	10.1007/s12038-010-0043-1
通讯作者	Zang, Qing-Wei
英文摘要	Plant cysteine protease (CP) genes are induced by abiotic stresses such as drought, yet their functions remain largely unknown. We isolated the full-length cDNA encoding a Triticum aestivum CP gene, designated TaCP, from wheat by the rapid amplification of cDNA ends (RACE) method. Sequence analysis revealed that TaCP contains an open reading frame encoding a protein of 362 amino acids, which is 96% identical to barley cysteine protease HvSF42. The TaCP transcript level in wheat seedlings was upregulated during polyethylene glycol (PEG) stress, with a peak appearing around 12 h after treatment. TaCP expression level increased rapidly with NaCl treatment at 48 h. TaCP responded strongly to low temperature (4A degrees C) treatment from 1 h post-treatment and reached a peak of about 40-fold at 72 h. However, it showed only a very slight response to abscisic acid (ABA). More than one copy of TaCP was present in each of the three genomes of hexaploid wheat and its diploid donors. TaCP fused with green fluorescent protein (GFP) was located in the plasma membrane of onion epidermis cells. Transgenic Arabidopsis plants overexpressing TaCP showed stronger drought tolerance and higher CP activity under water-stressed conditions than wild-type Arabidopsis plants. The results suggest that TaCP plays a role in tolerance to water deficit.
学科主题	Biology ; BIOLOGY
语种	英语
出版者	INDIAN ACAD SCIENCES
WOS记录号	WOS:000282596600008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5403]
专题	作物科学研究所
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Minist Agr, Key Lab Crop Germplasm & Biotechnol,Natl Key Faci, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zang, Qing-Wei,Wang, Cai-Xiang,Li, Xu-Yan,et al. Isolation and characterization of a gene encoding a polyethylene glycol-induced cysteine protease in common wheat[J]. JOURNAL OF BIOSCIENCES,2010,35(3):379-388.
APA	Zang, Qing-Wei.,Wang, Cai-Xiang.,Li, Xu-Yan.,Guo, Zhi-Ai.,Jing, Rui-Lian.,...&Chang, Xiao-Ping.(2010).Isolation and characterization of a gene encoding a polyethylene glycol-induced cysteine protease in common wheat.JOURNAL OF BIOSCIENCES,35(3),379-388.
MLA	Zang, Qing-Wei,et al."Isolation and characterization of a gene encoding a polyethylene glycol-induced cysteine protease in common wheat".JOURNAL OF BIOSCIENCES 35.3(2010):379-388.