Cloning and Expression Analysis of the Soybean CO-Like Gene GmCOL9

doi:10.1007/s11105-010-0240-y

CORC > 中国农业科学院 > 作物科学研究所

	Cloning and Expression Analysis of the Soybean CO-Like Gene GmCOL9
	Huang, Guowen 3; Ma, Jinghua 2; Han, Yuzhen 3; Chen, Xinjian 2; Fu, Yong-Fu 1
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2011
卷号	29 期号:2 页码:352-359
关键词	CONSTANS-Like Soybean Gene expression
ISSN号	0735-9640
DOI	10.1007/s11105-010-0240-y
通讯作者	Huang, Guowen
英文摘要	Photoperiod is one of the important environmental triggers of flowering, and the CONSTANS (CO) gene is a key regulator of this pathway in the model plant Arabidopsis thaliana. CO-like genes are present in many plant species. We describe here the isolation of the CO homolog Glycine max CONSTANS-like 9 (GmCOL9) from the soybean cultivar Kennong18. Sequence comparisons showed that the closest A. thaliana gene to GmCOL9 is COL13. The expression of GmCOL9 was investigated using quantitative real-time reverse transcriptase polymerase chain reaction, which showed that GmCOL9 was not regulated by a circadian mechanism, since its expression oscillated at a cycle of 12 h instead of 24 h. GmCOL9 expression was influenced by the developmental stage of the plant and varied between tissue/organs. The expression of GmCOL9 was concentrated in the unifoliolate leaves during the vegetative phase of growth and in the stem during the reproductive phase. However, traces of expression were also present throughout the plant, indicating that GmCOL9 may be a multifunctional gene in the context of the development of the soybean plant.
学科主题	Biochemical Research Methods ; Plant Sciences ; BIOCHEMICAL RESEARCH METHODS ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000288712400009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5303]
专题	作物科学研究所
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resource & Genet Improve, Beijing 100081, Peoples R China 2.Henan Agr Univ, Coll Life Sci, Zhengzhou 450002, Peoples R China 3.China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China
推荐引用方式 GB/T 7714	Huang, Guowen,Ma, Jinghua,Han, Yuzhen,et al. Cloning and Expression Analysis of the Soybean CO-Like Gene GmCOL9[J]. PLANT MOLECULAR BIOLOGY REPORTER,2011,29(2):352-359.
APA	Huang, Guowen,Ma, Jinghua,Han, Yuzhen,Chen, Xinjian,&Fu, Yong-Fu.(2011).Cloning and Expression Analysis of the Soybean CO-Like Gene GmCOL9.PLANT MOLECULAR BIOLOGY REPORTER,29(2),352-359.
MLA	Huang, Guowen,et al."Cloning and Expression Analysis of the Soybean CO-Like Gene GmCOL9".PLANT MOLECULAR BIOLOGY REPORTER 29.2(2011):352-359.